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MMP 9 ELISA Kit
MMP9
Reaktivität: Pferd
Colorimetric
Sandwich ELISA
49-12.000 pg/mL
Cell Culture Supernatant, Plasma, Serum
Produktnummer ABIN2703360
Produktdetails MMP 9 ELISA Kit
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Target
Alle MMP 9 (MMP9) ELISA Kits anzeigen
MMP 9 (MMP9)
(Matrix Metallopeptidase 9 (Gelatinase B, 92kDa Gelatinase, 92kDa Type IV Collagenase) (MMP9))
Reaktivität
Alle Reaktivitäten für MMP 9 ELISA Kits
Pferd
Nachweismethode
Colorimetric
Methodentyp
Sandwich ELISA
Detektionsbereich
49-12.000 pg/mL
Untere Nachweisgrenze
49 pg/mL
Applikation
ELISA
Verwendungszweck
Equine MMP-9 ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.
Proben
Cell Culture Supernatant, Plasma, Serum
Analytische Methode
Quantitative
Spezifität
The antibody pair provided in this kit recognizes Equine MMP-9
Sensitivität
49 pg/mL
Produktmerkmale
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Bestandteile
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Benötigtes Material
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Anwendungsinformationen
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Applikationshinweise
Recommended Dilution for serum and plasma samples3 fold
Probenmenge
100 μL
Plattentyp
Pre-coated
Protokoll
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Handhabung
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Lagerung
-20 °C
Informationen zur Lagerung
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Haltbarkeit
6 months
Details zu MMP 9
(ausblenden)
Target
Alle MMP 9 (MMP9) ELISA Kits anzeigen
MMP 9 (MMP9)
(Matrix Metallopeptidase 9 (Gelatinase B, 92kDa Gelatinase, 92kDa Type IV Collagenase) (MMP9))
Andere Bezeichnung
MMP9 (MMP9 Produkte )
Synonyme
CLG4B ELISA Kit, GELB ELISA Kit, MANDP2 ELISA Kit, MMP-9 ELISA Kit, AW743869 ELISA Kit, B/MMP9 ELISA Kit, Clg4b ELISA Kit, pro-MMP-9 ELISA Kit, mmp-9 ELISA Kit, fgMMP-9 ELISA Kit, mmp9 ELISA Kit, ZFMMP-9 ELISA Kit, wu:fb02g06 ELISA Kit, wu:fb07b05 ELISA Kit, wu:fi98c09 ELISA Kit, wu:fj05a08 ELISA Kit, zgc:64165 ELISA Kit, clg4b ELISA Kit, gelb ELISA Kit, mandp2 ELISA Kit, matrix metallopeptidase 9 ELISA Kit, collagenase ELISA Kit, matrix metalloproteinase 9 ELISA Kit, matrix metallopeptidase 9 S homeolog ELISA Kit, MMP9 ELISA Kit, RB3913 ELISA Kit, BPSS0666 ELISA Kit, Sbal_3732 ELISA Kit, Bcer98_0486 ELISA Kit, Shew185_0630 ELISA Kit, Shal_3056 ELISA Kit, Sbal195_0657 ELISA Kit, Lbys_3550 ELISA Kit, Palpr_2084 ELISA Kit, Mmp9 ELISA Kit, mmp9 ELISA Kit, mmp9.S ELISA Kit
Pathways
Cellular Response to Molecule of Bacterial Origin , Positive Regulation of Immune Effector Process , CXCR4-mediated Signaling Events
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