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IL1F9 ELISA Kit
IL1F9
Reaktivität: Human
Colorimetric
Sandwich ELISA
13-3000 pg/mL
Cell Culture Supernatant, Plasma, Serum
Produktnummer ABIN2703199
Produktdetails IL1F9 ELISA Kit
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Target
Alle IL1F9 ELISA Kits anzeigen
IL1F9
(Interleukin 1 Family Member 9 (IL1F9))
Reaktivität
Alle Reaktivitäten für IL1F9 ELISA Kits
Human
Nachweismethode
Colorimetric
Methodentyp
Sandwich ELISA
Detektionsbereich
13-3000 pg/mL
Untere Nachweisgrenze
13 pg/mL
Applikation
ELISA
Verwendungszweck
Human IL-36 gamma (IL-1 F9) ELISA Kit for cell culture supernatants, plasma, and serum samples.
Proben
Plasma, Cell Culture Supernatant, Serum
Analytische Methode
Quantitative
Spezifität
This ELISA kit shows no cross-reactivity with the following cytokines tested: human IL-1F1 (IL-1 alpha), IL-1F2 (IL-1 beta), IL-1F3 (IL-1ra), IL-1F4 (IL-18), IL-1F5 (IL-36ra), IL-1F6 (IL-36 alpha), IL-1F7 (IL-37), IL-1F8 (IL-36 beta), IL-1F10 (IL-38), IL-1F11 (IL-33)
Sensitivität
13 pg/mL
Produktmerkmale
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Bestandteile
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Benötigtes Material
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Anwendungsinformationen
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Applikationshinweise
Recommended Dilution for serum and plasma samples2 fold
Probenmenge
100 μL
Plattentyp
Pre-coated
Protokoll
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Handhabung
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Lagerung
-20 °C
Informationen zur Lagerung
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Haltbarkeit
6 months
Details zu IL1F9
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Target
Alle IL1F9 ELISA Kits anzeigen
IL1F9
(Interleukin 1 Family Member 9 (IL1F9))
Andere Bezeichnung
IL-1F9 (IL1F9 Produkte )
Synonyme
IL-1F9 ELISA Kit, IL-1H1 ELISA Kit, IL-1RP2 ELISA Kit, IL1E ELISA Kit, IL1F9 ELISA Kit, IL1H1 ELISA Kit, IL1RP2 ELISA Kit, Il36g ELISA Kit, Il1f9 ELISA Kit, RGD1563019 ELISA Kit, IL36G ELISA Kit, interleukin 36 gamma ELISA Kit, interleukin 1 family, member 9 ELISA Kit, interleukin 36, gamma ELISA Kit, interleukin-36 gamma ELISA Kit, IL36G ELISA Kit, Il1f9 ELISA Kit, Il36g ELISA Kit, LOC100065031 ELISA Kit
Hintergrund
Gene Names: IL36G IL1E IL1F9 IL1H1 IL1RP2 UNQ2456/PRO5737 Protein names: Interleukin-36 gamma (IL-1-related protein 2) (IL-1RP2) (Interleukin-1 epsilon) (IL-1 epsilon) (Interleukin-1 family member 9) (IL-1F9) (Interleukin-1 homolog 1) (IL-1H1)
Gen-ID
56300
UniProt
Q9NZH8
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