SPINT2 ELISA Kit

Produktnummer ABIN2703071

Produktdetails SPINT2 ELISA Kit

Target: SPINT2 (serine Peptidase Inhibitor, Kunitz Type, 2 (SPINT2))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 3-800 pg/mL

Untere Nachweisgrenze: 3 pg/mL

Applikation: ELISA

Verwendungszweck: Human HAI-2 ELISA Kit for cell culture supernatants, EDTA and/or Citrate treated plasma, and serum samples. Heparin is not recommended as an anticoagulant for use in this assay.

Proben: Cell Culture Supernatant, Plasma, Serum

Analytische Methode: Quantitative

Spezifität: This ELISA antibody pair detects human HAI-2, less than 0.1 % cross-reactivity with human HAI-1 and mouse HAI-2B is observed

Sensitivität: 3 pg/mL

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Recommended Dilution for serum and plasma samples2 fold

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Aufbereitung der Proben:

Collect plasma using EDTA and/or Citrate as anticoagulants. Heparin is not recommended as an anticoagulant for use in this assay.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Details zu SPINT2

Target: SPINT2 (serine Peptidase Inhibitor, Kunitz Type, 2 (SPINT2))

Andere Bezeichnung: HAI-2 (SPINT2 Produkte)

Synonyme: HAI-2 ELISA Kit, Pb ELISA Kit, DIAR3 ELISA Kit, HAI2 ELISA Kit, Kop ELISA Kit, PB ELISA Kit, AL024025 ELISA Kit, C76321 ELISA Kit, serine peptidase inhibitor, Kunitz type, 2 ELISA Kit, serine peptidase inhibitor, Kunitz type 2 ELISA Kit, serine protease inhibitor, Kunitz type 2 ELISA Kit, Spint2 ELISA Kit, SPINT2 ELISA Kit

Hintergrund: Gene Names: SPINT2 HAI2 KOP
Protein names: Kunitz-type protease inhibitor 2 (Hepatocyte growth factor activator inhibitor type 2) (HAI-2) (Placental bikunin)

Gen-ID: 10653

UniProt: O43291