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SPINT1 ELISA Kit
SPINT1
Reaktivität: Human
Colorimetric
Sandwich ELISA
0.8-200 ng/mL
Cell Culture Supernatant, Plasma, Serum
Produktnummer ABIN2703070
Produktdetails SPINT1 ELISA Kit
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Target
Alle SPINT1 ELISA Kits anzeigen
SPINT1
(serine Peptidase Inhibitor, Kunitz Type 1 (SPINT1))
Reaktivität
Alle Reaktivitäten für SPINT1 ELISA Kits
Human
Nachweismethode
Colorimetric
Methodentyp
Sandwich ELISA
Detektionsbereich
0.8-200 ng/mL
Untere Nachweisgrenze
0.8 ng/mL
Applikation
ELISA
Verwendungszweck
Human HAI-1 ELISA Kit for cell culture supernatants, plasma, and serum samples.
Proben
Cell Culture Supernatant, Plasma, Serum
Analytische Methode
Quantitative
Spezifität
This ELISA antibody pair detects human HAI-1, no cross-reactivity with mouse HAI-1, human APP, GASP-1, GASP-2, HAI-2A, TFPI, TFPI-2 is observed.
Sensitivität
0.8 ng/mL
Produktmerkmale
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Bestandteile
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Benötigtes Material
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Anwendungsinformationen
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Applikationshinweise
Recommended Dilution for serum and plasma samples2 fold
Probenmenge
100 μL
Plattentyp
Pre-coated
Protokoll
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Handhabung
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Lagerung
-20 °C
Informationen zur Lagerung
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Haltbarkeit
6 months
Details zu SPINT1
(ausblenden)
Target
Alle SPINT1 ELISA Kits anzeigen
SPINT1
(serine Peptidase Inhibitor, Kunitz Type 1 (SPINT1))
Andere Bezeichnung
HAI-1 (SPINT1 Produkte )
Synonyme
HAI-1 ELISA Kit, HAI ELISA Kit, HAI1 ELISA Kit, MANSC2 ELISA Kit, cb376 ELISA Kit, sb:cb376 ELISA Kit, spint1l ELISA Kit, zgc:64075 ELISA Kit, serine peptidase inhibitor, Kunitz type 1 ELISA Kit, serine protease inhibitor, Kunitz type 1 ELISA Kit, serine peptidase inhibitor, Kunitz type 1 b ELISA Kit, serine peptidase inhibitor, Kunitz type 1 a ELISA Kit, SPINT1 ELISA Kit, Spint1 ELISA Kit, spint1b ELISA Kit, spint1a ELISA Kit
Hintergrund
Gene Names: SPINT1 HAI1 UNQ223/PRO256 Protein names: Kunitz-type protease inhibitor 1 (Hepatocyte growth factor activator inhibitor type 1) (HAI-1)
Gen-ID
6692
UniProt
O43278
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