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FABP3 ELISA Kit
FABP3
Reaktivität: Human
Colorimetric
Sandwich ELISA
1.5-200 ng/mL
Cell Culture Supernatant, Plasma, Serum
Produktnummer ABIN2702991
Produktdetails FABP3 ELISA Kit
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Target
Alle FABP3 ELISA Kits anzeigen
FABP3
(Fatty Acid Binding Protein 3, Muscle and Heart (FABP3))
Reaktivität
Alle Reaktivitäten für FABP3 ELISA Kits
Human
Nachweismethode
Colorimetric
Methodentyp
Sandwich ELISA
Detektionsbereich
1.5-200 ng/mL
Untere Nachweisgrenze
1.5 ng/mL
Applikation
ELISA
Verwendungszweck
Human FABP3 ELISA Kit for cell culture supernatants, plasma, and serum samples.
Proben
Cell Culture Supernatant, Plasma, Serum
Analytische Methode
Quantitative
Spezifität
This ELISA antibody pair detects human FABP3. Other species not determined.
Sensitivität
1.5 ng/mL
Produktmerkmale
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Bestandteile
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Benötigtes Material
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Anwendungsinformationen
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Applikationshinweise
Recommended Dilution for serum and plasma samples2 fold
Probenmenge
100 μL
Plattentyp
Pre-coated
Protokoll
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Handhabung
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Lagerung
-20 °C
Informationen zur Lagerung
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Haltbarkeit
6 months
Details zu FABP3
(ausblenden)
Target
Alle FABP3 ELISA Kits anzeigen
FABP3
(Fatty Acid Binding Protein 3, Muscle and Heart (FABP3))
Andere Bezeichnung
FABP-3 (FABP3 Produkte )
Synonyme
Fabph-1 ELISA Kit, Fabph-4 ELISA Kit, Fabph1 ELISA Kit, Fabph4 ELISA Kit, H-FABP ELISA Kit, Mdgi ELISA Kit, FABP11 ELISA Kit, M-FABP ELISA Kit, MDGI ELISA Kit, O-FABP ELISA Kit, FABP ELISA Kit, FABP-3 ELISA Kit, fabp3 ELISA Kit, fatty acid binding protein 3 ELISA Kit, fatty acid binding protein 3, muscle and heart ELISA Kit, FABP3 ELISA Kit, Fabp3 ELISA Kit, fabp3 ELISA Kit
Hintergrund
Gene Names: FABP3 FABP11 MDGI Protein names: Fatty acid-binding protein, heart (Fatty acid-binding protein 3) (Heart-type fatty acid-binding protein) (H-FABP) (Mammary-derived growth inhibitor) (MDGI) (Muscle fatty acid-binding protein) (M-FABP)
Gen-ID
2170
UniProt
P05413
Pathways
Monocarboxylic Acid Catabolic Process
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