2-ThioBarbituric Acid Reactive Substances (TBARS) ELISA Kit
Reaktivität: Chemical
Colorimetric
Plasma, Serum, Urine
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- Target
- 2-ThioBarbituric Acid Reactive Substances (TBARS)
- Reaktivität
- Chemical
- Nachweismethode
- Colorimetric
- Applikation
- ELISA
- Proben
- Serum, Plasma, Urine
- Sensitivität
- 0.5 μM
- Produktmerkmale
- 2-ThioBarbituric Acid Reactive Substances (TBARS) are naturally present in biological specimens and include lipid hydroperoxides and aldehydes which increase in concentration as a response to oxidative stress.1,2 TBARS assay values are usually reported in malonaldehyde (malondialdehyde, MDA) equivalents, a compound that results from the decomposition of polyunsaturated fatty acid lipid peroxides. The TBARS assay is a well-recognized, established method for quantifying these lipid peroxides, although it has been criticized for its reactivity towards other compounds other than MDA.3 This TBARS Cuvette Assay Kit offers the researcher a straightforward, reproducible and consistent method for analyzing urine for lipid peroxidation products.
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- Applikationshinweise
- Optimal working dilution should be determined by the investigator.
- Probenmenge
- 100 μL
- Testdauer
- 0.5 h
- Plattentyp
- Pre-coated
- Protokoll
- This TBARS Cuvette Assay Kit is based on the reaction of a chromogenic reagent, 2-thiobarbituric acid, with MDA at 25 °C. One molecule of MDA reacts with 2 Molecules of 2-thiobarbituric acid via a Knoevenagel-type condensation to yield a chromophore with absorbance maximum at 532 nm,
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Lagerung
- 4 °C
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- Target
- 2-ThioBarbituric Acid Reactive Substances (TBARS)
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