IL-1 beta ELISA Kit

Produktnummer ABIN2648758

Maus IL-1 beta ELISA-Kit Colorimetric-Assay zur Quantifizierung von Maus IL-1 beta.

Kurzübersicht für IL-1 beta ELISA Kit (ABIN2648758)

Target: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Reaktivität: Maus

Nachweismethode: Colorimetric

Applikation: ELISA

Proben: Serum, Plasma, Cell Culture Cells

Produktdetails IL-1 beta ELISA Kit

Verwendungszweck: Mouse Interleukin 1 Beta (IL-1β) ELISA Assay Kit (enzyme-linked immunoassay kit) is intended for the quantitative determination of mouse γ-interferon (IL-1β) concentrations in cell culture supernates, serum, and plasma.

Analytische Methode: Quantitative

Sensitivität: 7 pg/ml

Produktmerkmale: Mouse Interleukin 1 Beta (IL-1β) ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Probenmenge: 100 μL

Testdauer: 3.5 h

Plattentyp: Pre-coated

Protokoll: Mouse Interleukin 1 Beta (IL-1β) ELISA Assay Kit employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for Interleukin 1 Beta (IL-1β) has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Interleukin 1 Beta (IL-1β) present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for Interleukin 1 Beta (IL-1β) is added to the wells and binds to the combination of capture antibody-IL-1β in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A colored product is formed in proportion to the amount of Interleukin 1 Beta (IL-1β) present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven Interleukin 1 Beta (IL-1β) standard dilutions and Interleukin 1 Beta (IL-1β) sample concentration determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Details zu IL-1 beta

Target: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Andere Bezeichnung: IL-1beta

Pathways: NF-kappaB Signalweg, Interferon-gamma Pathway, TLR Signalweg, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Autophagie, Cancer Immune Checkpoints, Inflammasome