SRD5A1 ELISA Kit

Produktnummer ABIN2044609

Produktdetails SRD5A1 ELISA Kit

Target: SRD5A1 (Steroid-5-alpha-Reductase, alpha Polypeptide 1 (3-Oxo-5 alpha-Steroid delta 4-Dehydrogenase alpha 1) (SRD5A1))

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.15-10 ng/mL

Untere Nachweisgrenze: 0.15 ng/mL

Applikation: ELISA

Marke: BioAssay™

Sensitivität: < 0.058 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Steroid 5 Alpha Reductase 1 (SRD5a1) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Steroid 5 Alpha Reductase 1 (SRD5a1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Steroid 5 Alpha Reductase 1 (SRD5a1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Steroid 5 Alpha Reductase 1 (SRD5a1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Steroid 5 Alpha Reductase 1 (SRD5a1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu SRD5A1

Target: SRD5A1 (Steroid-5-alpha-Reductase, alpha Polypeptide 1 (3-Oxo-5 alpha-Steroid delta 4-Dehydrogenase alpha 1) (SRD5A1))

Andere Bezeichnung: Steroid 5 alpha Reductase 1 (SRD5a1) (SRD5A1 Produkte)

Synonyme: zgc:153768 ELISA Kit, 0610031P22Rik ELISA Kit, 4930435F02Rik ELISA Kit, S5AR 1 ELISA Kit, Srd5a-1 ELISA Kit, steroid 5 alpha-reductase 1 ELISA Kit, steroid-5-alpha-reductase, alpha polypeptide 1 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 1) ELISA Kit, 3-oxo-5-alpha-steroid 4-dehydrogenase 1 ELISA Kit, SRD5A1 ELISA Kit, srd5a1 ELISA Kit, Bm1_06570 ELISA Kit, CC1G_14953 ELISA Kit, Srd5a1 ELISA Kit

Pathways: Metabolism of Steroid Hormones and Vitamin D, Steroid Hormone Biosynthesis, Response to Growth Hormone Stimulus, C21-Steroid Hormone Metabolic Process