Sema4a ELISA Kit

Produktnummer ABIN2044517

Produktdetails Sema4a ELISA Kit

Target: Sema4a (Sema Domain, Immunoglobulin Domain (Ig), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 4A (Sema4a))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.31-20 ng/mL

Untere Nachweisgrenze: 0.31 ng/mL

Applikation: ELISA

Marke: BioAssay™

Sensitivität: < 0.131 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Semaphorin 4A (SEMA4A) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Semaphorin 4A (SEMA4A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Semaphorin 4A (SEMA4A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Semaphorin 4A (SEMA4A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Semaphorin 4A (SEMA4A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu Sema4a

Target: Sema4a (Sema Domain, Immunoglobulin Domain (Ig), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 4A (Sema4a))

Andere Bezeichnung: Semaphorin 4A (SEMA4A) (Sema4a Produkte)

Synonyme: SEMA4A ELISA Kit, CORD10 ELISA Kit, RP35 ELISA Kit, SEMAB ELISA Kit, SEMB ELISA Kit, AI132332 ELISA Kit, SemB ELISA Kit, Semab ELISA Kit, semaphorin 4A ELISA Kit, sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4A ELISA Kit, SEMA4A ELISA Kit, sema4a ELISA Kit, Sema4a ELISA Kit