MARCKS ELISA Kit

Produktnummer ABIN2044021

Produktdetails MARCKS ELISA Kit

Target: MARCKS (Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS))

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 1.56-100 ng/mL

Untere Nachweisgrenze: 1.56 ng/mL

Applikation: ELISA

Marke: BioAssay™

Sensitivität: < 0.55 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Myristoylated Alanine Rich Protein Kinase C Substrate (MARCKS) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Myristoylated Alanine Rich Protein Kinase C Substrate (MARCKS). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Myristoylated Alanine Rich Protein Kinase C Substrate (MARCKS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Myristoylated Alanine Rich Protein Kinase C Substrate (MARCKS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Myristoylated Alanine Rich Protein Kinase C Substrate (MARCKS) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu MARCKS

Target: MARCKS (Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS))

Andere Bezeichnung: Myristoylated Alanine Rich Protein Kinase C Substrate (MARCKS) (MARCKS Produkte)

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