HYAL1 ELISA Kit

Produktnummer ABIN2043723

Produktdetails HYAL1 ELISA Kit

Target: HYAL1 (Hyaluronidase-1 (HYAL1))

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 1.56-100 ng/mL

Untere Nachweisgrenze: 1.56 ng/mL

Applikation: ELISA

Marke: BioAssay™

Sensitivität: < 0.55 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Hyaluronoglucosaminidase 1 (HYAL1) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hyaluronoglucosaminidase 1 (HYAL1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Hyaluronoglucosaminidase 1 (HYAL1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hyaluronoglucosaminidase 1 (HYAL1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Hyaluronoglucosaminidase 1 (HYAL1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu HYAL1

Target: HYAL1 (Hyaluronidase-1 (HYAL1))

Andere Bezeichnung: Hyaluronoglucosaminidase 1 (HYAL1) (HYAL1 Produkte)

Synonyme: HYAL-1 ELISA Kit, LUCA1 ELISA Kit, NAT6 ELISA Kit, Hya1 ELISA Kit, Hyal-1 ELISA Kit, hyaluronoglucosaminidase 1 ELISA Kit, HYAL1 ELISA Kit, Hyal1 ELISA Kit

Pathways: Glycosaminoglycan Metabolic Process