IL-6 ELISA Kit

Produktnummer ABIN2043679

Human IL-6 ELISA Kit, Colorimetric Assay für die Quantifizierung von Human IL-6.

Kurzübersicht für IL-6 ELISA Kit (ABIN2043679)

Target: IL-6 (IL6) (Interleukin 6 (IL6))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.15-10 pg/mL

Applikation: ELISA

Produktdetails IL-6 ELISA Kit

Untere Nachweisgrenze: 0.15 pg/mL

Sensitivität: < 0.051 pg/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: High Sensitivity Interleukin 6 (IL6) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 6 (IL6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 6 (IL6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 6 (IL6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Interleukin 6 (IL6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu IL-6

Target: IL-6 (IL6) (Interleukin 6 (IL6))

Pathways: TLR Signalweg, Hormone Transport, Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Autophagie, Cell RedoxHomeostasis, Cancer Immune Checkpoints, Inflammasome