Hexosaminidase A ELISA Kit

Produktnummer ABIN2043641

Produktdetails Hexosaminidase A ELISA Kit

Target: Hexosaminidase A (HEXA)

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.15-10 ng/mL

Untere Nachweisgrenze: 0.15 ng/mL

Applikation: ELISA

Marke: BioAssay™

Sensitivität: < 0.051 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Hexosaminidase A Alpha (HEXa) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hexosaminidase A Alpha (HEXa). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Hexosaminidase A Alpha (HEXa). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hexosaminidase A Alpha (HEXa), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Hexosaminidase A Alpha (HEXa) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu Hexosaminidase A

Target: Hexosaminidase A (HEXA)

Andere Bezeichnung: Hexosaminidase A alpha (HEXa) (HEXA Produkte)

Synonyme: TSD ELISA Kit, fc04h11 ELISA Kit, wu:fc04h11 ELISA Kit, wu:fv09f06 ELISA Kit, si:dkey-35i22.2 ELISA Kit, HEXA ELISA Kit, Hex-1 ELISA Kit, zgc:112084 ELISA Kit, hexosaminidase subunit alpha ELISA Kit, hexosaminidase B (beta polypeptide) ELISA Kit, CUGBP Elav-like family member 6 ELISA Kit, hexosaminidase A ELISA Kit, hexosaminidase A (alpha polypeptide) ELISA Kit, beta-hexosaminidase subunit alpha-like ELISA Kit, HEXA ELISA Kit, hexb ELISA Kit, CELF6 ELISA Kit, Hexa ELISA Kit, hexa ELISA Kit, LOC100228061 ELISA Kit

Pathways: Sensory Perception of Sound, Glycosaminoglycan Metabolic Process