CYP11B1 ELISA Kit

Produktnummer ABIN2043267

Produktdetails CYP11B1 ELISA Kit

Target: CYP11B1 (Cytochrome P450, Family 11, Subfamily B, Polypeptide 1 (CYP11B1))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.15-10 ng/mL

Untere Nachweisgrenze: 0.15 ng/mL

Applikation: ELISA

Marke: BioAssay™

Sensitivität: < 0.064 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Cytochrome P450 11B1 (CYP11B1) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytochrome P450 11B1 (CYP11B1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cytochrome P450 11B1 (CYP11B1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cytochrome P450 11B1 (CYP11B1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Cytochrome P450 11B1 (CYP11B1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu CYP11B1

Target: CYP11B1 (Cytochrome P450, Family 11, Subfamily B, Polypeptide 1 (CYP11B1))

Andere Bezeichnung: Cytochrome P450 11B1 (CYP11B1) (CYP11B1 Produkte)

Synonyme: CPN1 ELISA Kit, CYP11B ELISA Kit, FHI ELISA Kit, P450C11 ELISA Kit, AA389061 ELISA Kit, Cyp11b ELISA Kit, Cyp11b-1 ELISA Kit, Cp11ba ELISA Kit, RATCP11BA ELISA Kit, P450c11 ELISA Kit, cytochrome P450, family 11, subfamily B, polypeptide 1 ELISA Kit, cytochrome P450 family 11 subfamily B member 1 ELISA Kit, cytochrome P450, family 11, subfamily b, polypeptide 1 ELISA Kit, cytochrome P450, subfamily XI B, polypeptide 1 ELISA Kit, CYP11B1 ELISA Kit, Cyp11b1 ELISA Kit

Pathways: Metabolism of Steroid Hormones and Vitamin D, Steroid Hormone Biosynthesis, Regulation of Systemic Arterial Blood Pressure by Hormones, C21-Steroid Hormone Metabolic Process, Carbohydrate Homeostasis