Cyclin Y ELISA Kit

Produktnummer ABIN2043253

Produktdetails Cyclin Y ELISA Kit

Target: Cyclin Y (CCNY)

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 78.12-5000 pg/mL

Untere Nachweisgrenze: 78.12 pg/mL

Applikation: ELISA

Marke: BioAssay™

Sensitivität: < 29 pg/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Cyclin Y (CCNY) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cyclin Y (CCNY). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cyclin Y (CCNY). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cyclin Y (CCNY), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Cyclin Y (CCNY) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu Cyclin Y

Target: Cyclin Y (CCNY)

Abstract: CCNY Produkte

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