ATP5J ELISA Kit

Produktnummer ABIN2043233

Produktdetails ATP5J ELISA Kit

Target: ATP5J (ATP Synthase, H+ Transporting, Mitochondrial F0 Complex, Subunit F6 (ATP5J))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.15-10 ng/mL

Untere Nachweisgrenze: 0.15 ng/mL

Applikation: ELISA

Marke: BioAssay™

Sensitivität: < 0.068 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Coupling Factor 6 (CF6) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Coupling Factor 6 (CF6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Coupling Factor 6 (CF6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Coupling Factor 6 (CF6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Coupling Factor 6 (CF6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu ATP5J

Target: ATP5J (ATP Synthase, H+ Transporting, Mitochondrial F0 Complex, Subunit F6 (ATP5J))

Andere Bezeichnung: Coupling Factor 6 (CF6) (ATP5J Produkte)

Synonyme: zgc:77541 ELISA Kit, wu:fa66e04 ELISA Kit, ATP5 ELISA Kit, ATP5A ELISA Kit, ATPM ELISA Kit, CF6 ELISA Kit, F6 ELISA Kit, ATP synthase, H+ transporting, mitochondrial Fo complex, subunit F6 ELISA Kit, ATP synthase, H+ transporting, mitochondrial Fo complex subunit F6 ELISA Kit, ATP synthase, H+ transporting, mitochondrial Fo complex subunit F6 L homeolog ELISA Kit, ATP synthase, H+ transporting, mitochondrial F0 complex, subunit F6 ELISA Kit, ATP synthase, H+ transporting, mitochondrial F0 complex, subunit F ELISA Kit, atp5j ELISA Kit, ATP5J ELISA Kit, atp5j.L ELISA Kit, Atp5j ELISA Kit

Pathways: Proton Transport, Ribonucleoside Biosynthetic Process