COL12A1 ELISA Kit

Produktnummer ABIN2043189

Produktdetails COL12A1 ELISA Kit

Target: COL12A1 (Collagen, Type XII, alpha 1 (COL12A1))

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.31-20 ng/mL

Untere Nachweisgrenze: 0.31 ng/mL

Applikation: ELISA

Marke: BioAssay™

Sensitivität: < 0.134 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Collagen Type XII (COL12) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Collagen Type XII (COL12). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Collagen Type XII (COL12). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Collagen Type XII (COL12), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Collagen Type XII (COL12) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu COL12A1

Target: COL12A1 (Collagen, Type XII, alpha 1 (COL12A1))

Andere Bezeichnung: Collagen Type XII (COL12) (COL12A1 Produkte)

Synonyme: BA209D8.1 ELISA Kit, COL12A1L ELISA Kit, DJ234P15.1 ELISA Kit, AW743884 ELISA Kit, COL12A1 ELISA Kit, col12a1 ELISA Kit, collagen type XII alpha 1 chain ELISA Kit, collagen, type XII, alpha 1 ELISA Kit, collagen, type XII, alpha 1a ELISA Kit, Cuticle collagen 12 ELISA Kit, COL12A1 ELISA Kit, Col12a1 ELISA Kit, col12a1a ELISA Kit, col12a1 ELISA Kit, col-12 ELISA Kit