ARSF ELISA Kit

Produktnummer ABIN2042947

Produktdetails ARSF ELISA Kit

Target: ARSF (Arylsulfatase F (ARSF))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.31-20 ng/mL

Untere Nachweisgrenze: 0.31 ng/mL

Applikation: ELISA

Marke: BioAssay™

Sensitivität: < 0.112 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Arylsulfatase F (ARSF) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Arylsulfatase F (ARSF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Arylsulfatase F (ARSF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Arylsulfatase F (ARSF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Arylsulfatase F (ARSF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu ARSF

Target: ARSF (Arylsulfatase F (ARSF))

Abstract: ARSF Produkte

Synonyme: ASF ELISA Kit, ARSF ELISA Kit, arylsulfatase F ELISA Kit, ARSF ELISA Kit