HSD11B2 ELISA Kit

Produktnummer ABIN2042729

Produktdetails HSD11B2 ELISA Kit

Target: HSD11B2 (Hydroxysteroid (11-Beta) Dehydrogenase 2 (HSD11B2))

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.78-50 ng/mL

Untere Nachweisgrenze: 0.78 ng/mL

Applikation: ELISA

Marke: BioAssay™

Sensitivität: < 0.27 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: 11-Beta-Hydroxysteroid Dehydrogenase Type 2 (HSD11b2) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to 11-Beta-Hydroxysteroid Dehydrogenase Type 2 (HSD11b2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to 11-Beta-Hydroxysteroid Dehydrogenase Type 2 (HSD11b2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain 11-Beta-Hydroxysteroid Dehydrogenase Type 2 (HSD11b2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of 11-Beta-Hydroxysteroid Dehydrogenase Type 2 (HSD11b2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu HSD11B2

Target: HSD11B2 (Hydroxysteroid (11-Beta) Dehydrogenase 2 (HSD11B2))

Andere Bezeichnung: 11-beta-Hydroxysteroid Dehydrogenase Type 2 (HSD11b2) (HSD11B2 Produkte)

Synonyme: MGC81883 ELISA Kit, 11-HSD2 ELISA Kit, AME ELISA Kit, AME1 ELISA Kit, HSD11K ELISA Kit, HSD2 ELISA Kit, SDR9C3 ELISA Kit, 11HSD2 ELISA Kit, HSD11B2 ELISA Kit, hydroxysteroid (11-beta) dehydrogenase 2 L homeolog ELISA Kit, hydroxysteroid 11-beta dehydrogenase 2 ELISA Kit, hsd11b2.L ELISA Kit, HSD11B2 ELISA Kit, Hsd11b2 ELISA Kit

Pathways: Steroid Hormone Biosynthesis, Regulation of Systemic Arterial Blood Pressure by Hormones