IL-1 beta ELISA Kit

Produktnummer ABIN2042514

Dieses Maus IL-1 beta ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Maus IL-1 beta zu quantifizieren.

Kurzübersicht für IL-1 beta ELISA Kit (ABIN2042514)

Target: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 12.5-800 pg/mL

Applikation: ELISA

Produktdetails IL-1 beta ELISA Kit

Untere Nachweisgrenze: 12.5 pg/mL

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 1 pg/mL

Bestandteile: 96-well plate pre-coated with anti-IL-1 beta, Mouse
Lyophilized recombinant IL-1 beta, Mouse standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Sandwich ELISA quantitative detection kit for Mouse IL-1 beta in cell culture supernates, serum and plasma (heparin, EDTA).Interleukin-1 ( IL-1 ) is a potent stimulator of bone resorption whose gene is mapped to 2q14, and has been implicated in the pathogenesis of high bone turnover and osteoporosis. IL-1 , a prominent microglia-derived cytokine, caused oligodendrocyte death in coculture with astrocytes and microglia, but not in pure culture of oligodendrocytes alone1. It also can cause nuclear export of a specific NCOR corepressor complex, resulting in derepression of a specific subset of nuclear factor-kappa-B (NFKB)-regulated genes2. Furthermore, Microenvironmental IL-1 and, to a lesser extent, IL-1 are required for in vivo angiogenesis and invasiveness of different tumor cells3. Additional, the cooperation of IL-1 and PDGFB induces contractile-to-synthetic phenotype modulation of human aortic smooth muscle cells in culture4. Moreover, the association with disease may be explained by the biologic properties of IL-1 , which is an important proinflammatory cytokine and a powerful inhibitor of gastric acid secretion.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu IL-1 beta

Target: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Andere Bezeichnung: Interleukin-1 beta (IL-1 Beta)

Gen-ID: 16176

UniProt: P10749

Pathways: NF-kappaB Signalweg, Interferon-gamma Pathway, TLR Signalweg, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Autophagie, Cancer Immune Checkpoints, Inflammasome