IgG ELISA Kit
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- Target Alle IgG ELISA Kits anzeigen
- IgG
- Bindungsspezifität
- Heavy & Light Chain
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Reaktivität
- Rind (Kuh)
- Nachweismethode
- Colorimetric
- Methodentyp
- Sandwich ELISA
- Detektionsbereich
- 10-2000 ng/mL
- Untere Nachweisgrenze
- 10 ng/mL
- Applikation
- ELISA
- Verwendungszweck
- Rapid quantification of bovine IgG in batches of purified antibodies, selection of low IgG sera
- Marke
- FastELISA®
- Proben
- Cell Culture Supernatant, Serum
- Analytische Methode
- Quantitative
- Spezifität
- The method enables the detection of bovine IgG.
- Produktmerkmale
- The bovine immunoglobulin quantification kit provides a rapid and easy method (one step ELISA) for the quantitative determination of bovine IgG in cell culture supernatants and serums.
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- Discover our top product IgG ELISA Kit
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- Kommentare
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One Step ELISA 45 min total time
IgG contaminations of about 20 ppm - Testdauer
- < 1 h
- Plattentyp
- Pre-coated
- Protokoll
- A polyclonal antibody specific to bovine IgG is pre-coated onto microwells. Samples and standards are pipetted into microwells and bovine IgG present in the sample are bound by the capture antibody. Then, an HRP (horseradish peroxidase) conjugated anti-bovine IgG (H+L) antibody is pipetted and incubated simultaneously with samples. After washing microwells in order to remove any non specific binding, the ready to use substrate solution (TMB) is added to microwells and color develops proportionally to the amount of bovine IgG in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.
- Aufbereitung der Reagenzien
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dilution
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Lagerung
- 4 °C
- Haltbarkeit
- 12 months
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- Target Alle IgG ELISA Kits anzeigen
- IgG
- Andere Bezeichnung
- Immunoglobulin G (IgG Produkte)
- Substanzklasse
- Antibody
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