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Maus IgG2a, kappa isotype control (DyLight 488)

FACS, WB, ICC, IHC (fro), IHC (p) Monoclonal MOPC-173 IgG2a kappa DyLight 488
Produktnummer ABIN7014020
  • Target Alle IgG2a, kappa Produkte
    IgG2a, kappa
    Wirt
    • 77
    • 65
    • 1
    Maus
    Klonalität
    • 141
    • 2
    Monoklonal
    Konjugat
    DyLight 488
    Applikation
    Flow Cytometry (FACS), Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Spezifität
    This mouse IgG2a monoclonal antibody (clone MOPC-173) reacts with an unknown epitope. It does not react with a variety of resting, activated, live, and fixed mouse, rat and human tissues.
    Keine Kreuzreaktivität
    Human, Maus, Ratte
    Aufreinigung
    Purified antibody is conjugated with DyLight® 488 NHS ester under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
    Immunogen
    The transplantable plasmacytoma MOPC-173 was induced by intraperitoneal injection of mineral oils into BALB/c mice.
    Klon
    • 46
    • 44
    • 23
    • 17
    • 5
    • 4
    • 1
    • 1
    • 1
    • 1
    MOPC-173
    Isotyp
    IgG2a kappa
  • Applikationshinweise
    Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 μg/mL of incubation mixture), change the conditions of your experiment to reduce the background.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Konzentration
    0.1 mg/mL
    Buffer
    Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C
    Informationen zur Lagerung
    Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
  • Target
    IgG2a, kappa
    Andere Bezeichnung
    IgG2a kappa (IgG2a, kappa Produkte)
    Substanzklasse
    Antibody
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