The mouse lineage panel has been designed to react with cells from the major hematopoietic cell lineages, such as T lymphocytes, B lymphocytes, monocytes/macrophages, granulocytes, NK cells, and erythrocytes. The PE Mouse Lineage Antibody Cocktail is designed for the flow cytometric identification of hematopoietic progenitors in mouse bone marrow. Components include anti-mouse CD3ε, clone 145-2C11, anti-mouse Ly-6G/Ly-6C, clone RB6-8C5, anti-mouse CD11b, clone M1/70, anti-mouse CD45R/B220, clone RA3-6B2, anti-mouse TER-119/Erythroid cells, clone Ter-119. PE Mouse Lineage Isotype Control Cocktail contains equivalent concentrations of isotype-matched negative control immunoglobulin. Materials provided: 1. PE anti-mCD3ε/ PE anti-mGr-1/ PE anti-mCD11b/ PE anti-mCD45R(B220)/ PE anti-mTer-119, 25 tests 2. PE Armenian hamster IgG/ PE Rat IgG2b/ PE Rat IgG2a isotype controls, 25 tests Materials not provided: Cell Staining Buffer (Cat. No. 420201)
Aufreinigung
The antibodies were purified by affinity chromatography, and conjugated with PE under optimal conditions. The solutions are free of unconjugated PE and unconjugated antibody.