anti-Maus Mu Opioid Receptor 1 Antikörper für Immunocytochemistry

Recommended Mu Opioid Receptor 1 Antibody

Antigen
Opioid Receptor, mu 1 (OPRM1) Antikörper
  • LMOR
  • M-OR-1
  • MOP
  • MOR
  • MOR1
  • OPRM
  • MOP-R
  • MOR-1
  • MOR-1O
  • Oprm
  • mor
  • muOR
  • MORA
  • Oprrm1
  • or2
  • ZFOR2
  • OPRM1
  • opioid receptor mu 1
  • opioid receptor, mu 1
  • outer membrane protein OprM
  • OPRM1
  • Oprm1
  • oprm1
  • oprM1
Reaktivität
Human, Maus
132
68
56
10
8
8
3
3
2
2
1
1
1
1
1
1
Wirt
Kaninchen
135
3
3
Klonalität
Polyklonal
Konjugat
Dieser Mu Opioid Receptor 1 Antikörper ist unkonjugiert
6
5
4
2
2
2
2
2
2
2
2
2
1
1
1
1
1
Applikation
ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
94
55
48
30
16
14
7
5
5
2
1
1
Optionen

Bilder

Produktnummer ABIN6257397
$ 390.77
Zzgl. Versandkosten $45.00
Relevance Score ABIN Application Konjugat Host Isotype Epitope Clonality References Details
1 ABIN2772816 ICC IHC Guinea Pig IgG Polyclonal 0
1 ABIN2772817 ICC IHC Guinea Pig IgG Polyclonal 0
0.31509113 ABIN250445 ICC IF IHC WB Guinea Pig C-Term Polyclonal 1
0.31509113 ABIN250447 ICC IHC (fro) IF IHC WB Rabbit C-Term Polyclonal 2

General

Antigen Opioid Receptor, mu 1 (OPRM1) Antikörper
Reaktivität Human, Maus
(132), (68), (56), (10), (8), (8), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1)
Wirt Kaninchen
(135), (3), (3)
Klonalität
Polyklonal
Konjugat Dieser Mu Opioid Receptor 1 Antikörper ist unkonjugiert
(6), (5), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Applikation ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
(94), (55), (48), (30), (16), (14), (7), (5), (5), (2), (1), (1)
Hersteller

Produktdetails anti-Mu Opioid Receptor 1 Antikörper

Target Details Mu Opioid Receptor 1 Anwendungsinformationen Handhabung Bilder
Spezifität OPRM1 Antibody detects endogenous levels of total OPRM1
Kreuzreaktivität Human, Maus
Reinigung The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
Immunogen A synthesized peptide
Isotyp IgG
Plasmids, Primers & others

Target Details Mu Opioid Receptor 1

Produktdetails anti-Mu Opioid Receptor 1 Antikörper Anwendungsinformationen Handhabung Bilder zurück nach oben
Antigen
Andere Bezeichnung OPRM1 (OPRM1 Antibody Abstract)
Hintergrund

Description: Receptor for endogenous opioids such as beta-endorphin and endomorphin. Receptor for natural and synthetic opioids including morphine, heroin, DAMGO, fentanyl, etorphine, buprenorphin and methadone (PubMed:7905839, PubMed:7957926, PubMed:7891175, PubMed:12589820, PubMed:9689128). Agonist binding to the receptor induces coupling to an inactive GDP-bound heterotrimeric G-protein complex and subsequent exchange of GDP for GTP in the G-protein alpha subunit leading to dissociation of the G-protein complex with the free GTP-bound G-protein alpha and the G-protein beta-gamma dimer activating downstream cellular effectors (PubMed:7905839). The agonist- and cell type-specific activity is predominantly coupled to pertussis toxin-sensitive G(i) and G(o) G alpha proteins, GNAI1, GNAI2, GNAI3 and GNAO1 isoforms Alpha-1 and Alpha-2, and to a lesser extent to pertussis toxin-insensitive G alpha proteins GNAZ and GNA15 (PubMed:12068084). They mediate an array of downstream cellular responses, including inhibition of adenylate cyclase activity and both N-type and L-type calcium channels, activation of inward rectifying potassium channels, mitogen-activated protein kinase (MAPK), phospholipase C (PLC), phosphoinositide/protein kinase (PKC), phosphoinositide 3-kinase (PI3K) and regulation of NF-kappa-B. Also couples to adenylate cyclase stimulatory G alpha proteins. The selective temporal coupling to G-proteins and subsequent signaling can be regulated by RGSZ proteins, such as RGS9, RGS17 and RGS4. Phosphorylation by members of the GPRK subfamily of Ser/Thr protein kinases and association with beta-arrestins is involved in short-term receptor desensitization. Beta-arrestins associate with the GPRK-phosphorylated receptor and uncouple it from the G-protein thus terminating signal transduction. The phosphorylated receptor is internalized through endocytosis via clathrin-coated pits which involves beta-arrestins. The activation of the ERK pathway occurs either in a G-protein-dependent or a beta-arrestin-dependent manner and is regulated by agonist-specific receptor phosphorylation. Acts as a class A G-protein coupled receptor (GPCR) which dissociates from beta-arrestin at or near the plasma membrane and undergoes rapid recycling. Receptor down-regulation pathways are varying with the agonist and occur dependent or independent of G-protein coupling. Endogenous ligands induce rapid desensitization, endocytosis and recycling whereas morphine induces only low desensitization and endocytosis. Heterooligomerization with other GPCRs can modulate agonist binding, signaling and trafficking properties. Involved in neurogenesis. Isoform 12 couples to GNAS and is proposed to be involved in excitatory effects (PubMed:20525224). Isoform 16 and isoform 17 do not bind agonists but may act through oligomerization with binding-competent OPRM1 isoforms and reduce their ligand binding activity (PubMed:16580639).

Gene: OPRM1

Molekulargewicht 48 kDa
Gen-ID 4988
UniProt P35372
Pathways cAMP Metabolic Process, Synaptic Membrane

Anwendungsinformationen

Produktdetails anti-Mu Opioid Receptor 1 Antikörper Target Details Mu Opioid Receptor 1 Handhabung Bilder zurück nach oben
Applikations-hinweise WB 1:500~1:1000 IHC: 1:50~1:200 IF 1:100-500 ELISA(peptide) 1:20000-1:40000
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-Mu Opioid Receptor 1 Antikörper Target Details Mu Opioid Receptor 1 Anwendungsinformationen Bilder zurück nach oben
Format Liquid
Konzentration 1 mg/mL
Buffer Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung Store at -20 °C.Stable for 12 months from date of receipt
Haltbarkeit 12 months

Bilder

Produktdetails anti-Mu Opioid Receptor 1 Antikörper Target Details Mu Opioid Receptor 1 Anwendungsinformationen Handhabung zurück nach oben
Bilder des Herstellers
Western Blotting (WB) image for anti-Opioid Receptor, mu 1 (OPRM1) antibody (ABIN6257397) Western blot analysis of extracts from HeLa cells, using OPRM1 antibody.
Immunofluorescence (fixed cells) (IF/ICC) image for anti-Opioid Receptor, mu 1 (OPRM1) antibody (ABIN6257397) ABIN6276107 staining HeLa cells by ICC/IF. Cells were fixed with PFA and permeabilize...
Immunohistochemistry (IHC) image for anti-Opioid Receptor, mu 1 (OPRM1) antibody (ABIN6257397) ABIN6276107 at 1/100 staining Human brain cancer tissue by IHC-P. The sample was form...
Western Blotting (WB) image for anti-Opioid Receptor, mu 1 (OPRM1) antibody (ABIN6257397) Western blot analysis of extracts from various samples, using OPRM1 Antibody. Lane 1:...