RFP Selector

Details zu Produkt Nr. ABIN5311509, Anbieter: Anmelden zum Anzeigen
Antigen
  • RFP
  • RNF76
  • tripartite motif containing 27
  • TRIM27
Reaktivität
Discosoma
Wirt
Camelid (Camelidae)
Applikation
Protein Complex Immunoprecipitation (Co-IP), Immunoprecipitation (IP), Purification (Purif), Chromatin Immunoprecipitation (ChIP), RNA-Binding Protein Immunoprecipitation (RIP)
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
Anmelden zum Anzeigen
Proben Cell Extracts
Spezifität The Antibody with Clone 2B12 recognizes most common red fluorescent proteins like mRFP, mCherry, dsRed and tdTomato. Does not cross-react with GFP or mTagBFP/mTagRFP derivatives.
Produktmerkmale RFP Selector is based on a high-affinity single-domain antibody (sdAb) that is covalently immobilized on 4 % cross-linked agarose beads. The innovative, oriented and selective attachment via a flexible linker guarantees a high accessibility of the sdAbs and largely eliminates batch-to-batch variations. Due to the single-chain nature of sdAbs and their covalent attachment, no "leakage" of light and heavy chains from IgGs is observed during elution with SDS sample buffer. RFP Selector thus features high affinity and superior capacity for RFP fusion proteins while showing negligible non-specific background. RFP Selector immobilizes a wide range of RFP derivatives. For details see our Product Specificity Chart below. RFP Selector is compatible not only with physiological buffers but also with high stringency buffers. RFP Selector thus provides great freedom to adjust the binding and washing conditions to the experimental needs.
Bestandteile 4 % cross-linked agarose (bead size 50-150 μm) with covalently immobilized single-domain antibody
Benötigtes Material wash buffers, columns, tubes
Bead Ligand Antibody
Bead Matrix Agarose beads
Bead Size 90 μm
Hintergrund Red fluorescent protein RFP and its derivates have become very popular and universal tools in cell biology. It is a monomeric protein and an excellent fusion partner for chimeric proteins. The possibility to fuse RFP to the N- or the C-terminus of the pro
Applikations-hinweise 200 μL slurry for up to 10 reactions
Kommentare

Capacity: > 4 μg RFP per μl of packed beads (= 2μl of slurry)

Beschränkungen Nur für Forschungszwecke einsetzbar
Buffer 50 % slurry in 20 % ethanol / PBS 0.09 % sodium azide
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung 4 °C
Bilder des Herstellers
Immunoprecipitation (IP) image for RFP Selector (ABIN5311509) A) Pull-down of mCherry from a mixture of GFP, mCherry and mTagBFP B) Immunoprecipi...