RAF1 Antikörper (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1)

Details for Product anti-RAF1 Antibody No. ABIN969559, Anbieter: Anmelden zum Anzeigen
Antigen
  • CRAF
  • NS5
  • Raf-1
  • c-Raf
  • c-raf
  • craf
  • raf1
  • RAF1
  • 6430402F14Rik
  • AA990557
  • BB129353
  • Craf1
  • D830050J10Rik
  • v-Raf
  • raf
  • cRaf
  • Raf-1 proto-oncogene, serine/threonine kinase
  • Raf-1 proto-oncogene, serine/threonine kinase a
  • v-raf-leukemia viral oncogene 1
  • Raf-1 proto-oncogene, serine/threonine kinase S homeolog
  • RAF1
  • Raf1
  • raf1a
  • raf1
  • raf1.S
Reaktivität
Human
858
442
413
25
23
17
16
16
8
8
8
5
4
4
2
2
1
1
Wirt
Maus
825
44
11
5
2
Klonalität (Klon)
Monoklonal ()
Konjugat
Dieser RAF1 Antikörper ist unkonjugiert
44
44
43
29
29
29
12
12
11
11
11
11
11
11
11
11
11
1
1
1
1
1
1
1
1
1
1
Applikation
Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
546
404
252
182
143
105
61
34
30
18
16
10
2
2
2
1
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Immunogen Purified recombinant fragment of human RAF1 expressed in E. Coli.
Klon 4G4
Isotyp IgG1
Reinigung Purified
Plasmids, Primers & others Plasmide, Primers & weitere RAF1 products on genomics-online (e.g. as negative or positive controls)
Antigen
Andere Bezeichnung RAF1 (RAF1 Antibody Abstract)
Hintergrund This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.
Gen-ID 5894
HGNC 5894
Pathways MAPK Signalweg, RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, Neurotrophin Signalübertragung, cAMP Metabolic Process, Stem Cell Maintenance, Hepatitis C, Autophagie, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling
Applikations-hinweise ELISA: 1:10000, WB: 1:500 - 1:2000, IHC: 1:200 - 1:1000, FCM: 1:200 - 1:400
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Buffer PBS with 0.05 % sodium azide
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung 4 °C,-20 °C
Informationen zur Lagerung 4°C, -20°C for long term storage
Bilder des Herstellers
ELISA image for anti-V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) antibody (ABIN969559) Figure 1: Red: Control Antigen (100 ng), Purple: Antigen (10ng), Green: Antigen (50 n...
Flow Cytometry (FACS) image for anti-V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) antibody (ABIN969559) Figure 2: Flow cytometric analysis of Hela cells using RAF1 mouse mAb (green) and neg...
Immunohistochemistry (IHC) image for anti-V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) antibody (ABIN969559) Figure 3: Immunohistochemical analysis of paraffin-embedded liver cancer tissues usin...
Western Blotting (WB) image for anti-V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) antibody (ABIN969559) Figure 4: Western blot analysis using RAF1 mouse mAb against Hela (1), A431 (2), Cos7...
Western Blotting (WB) image for anti-V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) antibody (ABIN969559) Figure 5: Western blot analysis using RAF1 mAb against human RAF1 (AA: 198-407) recom...
Produkt verwendet in: Hofmann, Heinrich, Radziwill, Radziwil, Moelling: "A short hairpin DNA analogous to miR-125b inhibits C-Raf expression, proliferation, and survival of breast cancer cells." in: Molecular cancer research : MCR, Vol. 7, Issue 10, pp. 1635-44, 2009 (PubMed).

Talmud, Drenos, Shah, Shah, Palmen, Verzilli, Gaunt, Pallas, Lovering, Li, Casas, Sofat, Kumari, Rodriguez, Johnson, Newhouse, Dominiczak, Samani, Caulfield, Sever, Stanton, Shields, Padmanabhan et al.: "Gene-centric association signals for lipids and apolipoproteins identified via the HumanCVD BeadChip. ..." in: American journal of human genetics, Vol. 85, Issue 5, pp. 628-42, 2009 (PubMed).

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