SLC9A3R1 Antikörper (AA 128-249)

Produktnummer ABIN968397

Produktdetails anti-SLC9A3R1 Antikörper

Target: SLC9A3R1 (Solute Carrier Family 9, Subfamily A (NHE3, Cation Proton Antiporter 3), Member 3 Regulator 1 (SLC9A3R1))

Bindungsspezifität: AA 128-249

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser SLC9A3R1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF)

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human EBP50 aa. 128-249

Klon: 6-EBP50

Isotyp: IgG1

Anwendungsinformationen

Kommentare:

Related Products: ABIN968536, ABIN967389

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20° C.

Referenzen für anti-SLC9A3R1 Antikörper (ABIN968397)

Glynne, Darling, Picot, Evans: "Epithelial inducible nitric-oxide synthase is an apical EBP50-binding protein that directs vectorial nitric oxide output." in: The Journal of biological chemistry, Vol. 277, Issue 36, pp. 33132-8, (2002) (PubMed).

Bretscher, Reczek, Berryman: "Ezrin: a protein requiring conformational activation to link microfilaments to the plasma membrane in the assembly of cell surface structures." in: Journal of cell science, Vol. 110 ( Pt 24), pp. 3011-8, (1998) (PubMed).

Reczek, Berryman, Bretscher: "Identification of EBP50: A PDZ-containing phosphoprotein that associates with members of the ezrin-radixin-moesin family." in: The Journal of cell biology, Vol. 139, Issue 1, pp. 169-79, (1998) (PubMed).

Reczek, Bretscher: "The carboxyl-terminal region of EBP50 binds to a site in the amino-terminal domain of ezrin that is masked in the dormant molecule." in: The Journal of biological chemistry, Vol. 273, Issue 29, pp. 18452-8, (1998) (PubMed).

Details zu SLC9A3R1

Target: SLC9A3R1 (Solute Carrier Family 9, Subfamily A (NHE3, Cation Proton Antiporter 3), Member 3 Regulator 1 (SLC9A3R1))

Andere Bezeichnung: EBP50 (SLC9A3R1 Produkte)

Synonyme: EBP50 antikoerper, NHERF antikoerper, NHERF-1 antikoerper, NHERF1 antikoerper, NPHLOP2 antikoerper, SLC9A3R1 antikoerper, cb895 antikoerper, slc9a3r2 antikoerper, wu:fd20a02 antikoerper, zgc:55541 antikoerper, zgc:77629 antikoerper, EBP-50 antikoerper, NHE-RF antikoerper, nherf antikoerper, nherf-1 antikoerper, nherf1 antikoerper, slc9a3r1 antikoerper, SLC9A3 regulator 1 antikoerper, microRNA 3615 antikoerper, solute carrier family 9, subfamily A (NHE3, cation proton antiporter 3), member 3 regulator 1a antikoerper, solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1 antikoerper, SLC9A3 regulator 1 S homeolog antikoerper, SLC9A3R1 antikoerper, MIR3615 antikoerper, slc9a3r1a antikoerper, Slc9a3r1 antikoerper, slc9a3r1.S antikoerper

Hintergrund: Integral plasma membrane proteins are stabilized by linkages to the cortical actin cytoskeleton, which structurally supports the membrane and contributes to processes such as endocytosis, exocytosis, and transmembrane signaling. The ERM (ezrin-radixin-moesin) family of proteins provides these structural linkages. The ERM proteins contain a 300-residue N-terminal domain, a 170-residue alpha-helical region, and a C-terminal 100-residue domain that contains F-actin binding sites. The N-terminal domain interacts with the cytoplasmic domain of CD44, the regulatory subunit of PKA, and the PDZ domain containing ERM-binding phosphoprotein (EBP-50). In polarized epithelial cells, EBP-50 links ezrin and the cytoplasmic regions of transmembrane proteins such as the cystic fibrosis transmembrane conductance regulator (CFTR) and the beta2-adrenergic receptor. EBP50 contains two PDZ domains followed by a C-terminal tail. It colocalizes with ezrin in apical microvilli and is also thought to interact with the Na+-H+ exchanger NHE3 to confer cAMP-mediated inhibition of Na+-H+ exchange. Thus, EBP-50 mediates membrane attachment to the cytoskeleton and may also function in the regulation of ion exchange. This antibody is routinely tested in western blot analysis.

Molekulargewicht: 50-53 kDa

Pathways: Proton Transport, Platelet-derived growth Factor Receptor Signaling, Negative Regulation of Transporter Activity, SARS-CoV-2 Protein Interaktom