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Bcl-2 Antikörper (AA 61-76)

Dieses Anti-Bcl-2-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von Bcl-2 in WB und IP. Geeignet für Human.
Produktnummer ABIN967317

Kurzübersicht für Bcl-2 Antikörper (AA 61-76) (ABIN967317)

Target

Alle Bcl-2 (BCL2) Antikörper anzeigen
Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))

Reaktivität

  • 472
  • 177
  • 171
  • 37
  • 26
  • 24
  • 16
  • 9
  • 8
  • 5
  • 5
  • 2
  • 1
  • 1
  • 1
  • 1
Human

Wirt

  • 362
  • 161
  • 4
  • 2
  • 1
  • 1
  • 1
Maus

Klonalität

  • 332
  • 199
Monoklonal

Konjugat

  • 236
  • 33
  • 32
  • 21
  • 19
  • 12
  • 11
  • 8
  • 8
  • 8
  • 8
  • 8
  • 8
  • 8
  • 8
  • 8
  • 8
  • 8
  • 8
  • 7
  • 6
  • 6
  • 6
  • 6
  • 6
  • 6
  • 5
  • 5
  • 5
  • 5
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Dieser Bcl-2 Antikörper ist unkonjugiert

Applikation

  • 412
  • 188
  • 188
  • 148
  • 104
  • 95
  • 83
  • 80
  • 58
  • 53
  • 48
  • 24
  • 23
  • 18
  • 8
  • 8
  • 8
  • 5
  • 4
  • 3
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB), Immunoprecipitation (IP)

Klon

4D7
  • Bindungsspezifität

    • 57
    • 39
    • 27
    • 23
    • 22
    • 21
    • 16
    • 16
    • 16
    • 16
    • 11
    • 11
    • 8
    • 8
    • 7
    • 7
    • 7
    • 7
    • 7
    • 5
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 61-76

    Marke

    BD Pharmingen™

    Produktmerkmale

    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. Please refer to us for technical protocols.
    3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

    Aufreinigung

    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

    Immunogen

    Human Bcl-2 aa. 61-76

    Isotyp

    IgG1
  • Applikationshinweise

    For immunohistochemistry of paraffin-embedded sections, the monoclonal mouse anti-human Bcl-2 (clone Bcl-2/100, ABIN967322) or clone 7/Bcl-2, (ABIN968025) is recommended. Jurkat control lysate [50 µg (1 µg/µl)] is provided as a western blot positive control (store lysate at -20°C). Additional control lysate (ABIN968537) is sold separately.

    Kommentare

    Related Products: ABIN968537

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Liquid

    Konzentration

    0.25 mg/mL

    Buffer

    Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Lagerung

    -20 °C

    Informationen zur Lagerung

    Store undiluted at -20°C.
  • Target

    Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))

    Andere Bezeichnung

    Bcl-2

    Hintergrund

    The bcl-2 gene was first discovered in t(14:18) follicular lymphomas. Translocation of bcl-2 sequences from chromosome 18 onto the transcriptionally active immunoglobulin locus at chromosome band 14q32 in B-cells deregulates bcl-2 gene expression, resulting in high levels of bcl-2 mRNA. Bcl-2 protein blocks apoptosis (programmed cell death), and thereby may contribute to tomorigenesis by prolonging cell survival rather than by accelerating the rate of cell proliferation. Abundant evidence implicates this protein in the suppression of apoptosis in many types of cells. Bcl-2 is an intracellular membrane protein and resides primarily in the nuclear envelope, outer mitochondrial membrane and endoplasmic reticulum. The reduced molecular weight of Bcl-2 is 26 kDa. Clone 4D7 recognizes human Bcl-2. A synthetic peptide containing amino acids 61-76 of the human Bcl-2 protein was used as immunogen. This peptide sequence differs from that of mouse Bcl-2 and hence 4D7 does not cross-react with mouse. 4D7 recognizes Bcl-2 as a 26 kDa band, however, additional higher molecular weight bands have been observed in some cell types. These bands may represent oligomers of Bcl-2, which have been reported to occur with members of the Bcl-2 family during the preparation of nonionic detergent extracts.

    Molekulargewicht

    26 kDa

    Pathways

    MAPK Signalweg, PI3K-Akt Signalweg, Apoptose, Caspase Kaskade in der Apoptose, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Skeletal Muscle Fiber Development, Autophagie, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling
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