Bcl-2 Antikörper (AA 41-54)

Produktnummer ABIN6940492

Der Maus Monoklonal anti-Bcl-2 Antikörper wird verwendet zum Nachweis von Bcl-2 in Proben von Human. Er wurde validiert für WB, IHC, IF und FACS.

Kurzübersicht für Bcl-2 Antikörper (AA 41-54) (ABIN6940492)

Target: Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Bcl-2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Klon: 100-D5

Produktdetails anti-Bcl-2 Antikörper

Bindungsspezifität: AA 41-54

Verwendungszweck: Bcl-2 (Apoptosis & Follicular Lymphoma Marker) Antibody

Spezifität: This antibody recognizes a protein of 25-26 kDa, identified as the bcl-2 lpha oncoprotein. It shows no cross-reaction with Bcl-x or Bax protein. Expression of bcl-2 lpha oncoprotein inhibits the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of bcl-2 lpha protein, whereas the normal or hyperplastic germinal centers are negative. Consequently, this antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies. It may also be used in distinguishing between those follicular lymphomas that express bcl-2 protein and the small number in which the neoplastic cells are bcl-2 negative.

Keine Kreuzreaktivität: Maus, Ratte (Rattus)

Aufreinigung: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: A synthetic peptide, aa41-54 (GAAPAPGIFSSQPG-Cys) of human Bcl-2 protein.

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: MCF-7, Jurkat, K562, HL-60 or HeLa cells. Tonsil or follicular lymphomas.

Known Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Tris with 1 mM EDTA Buffer, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu Bcl-2

Target: Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))

Andere Bezeichnung: BCL2

Hintergrund:

Apoptosis regulator Bcl-2, B-cell CLL/lymphoma-2

Jurkat or HeLa cells. Human tonsil or follicular lymphomas., MCF-7

Molekulargewicht: 25-26kDa

Gen-ID: 596, 150749

UniProt: P10415

Pathways: MAPK Signalweg, PI3K-Akt Signalweg, Apoptose, Caspase Kaskade in der Apoptose, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Skeletal Muscle Fiber Development, Autophagie, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling