NOS2 Antikörper (Nitric Oxide Synthase 2, Inducible) (C-Term)

Details for Product anti-NOS2 Antibody No. ABIN966678
Antigen
  • HEP-NOS
  • INOS
  • NOS
  • NOS2A
  • NOS-II
  • Nos-2
  • Nos2a
  • i-NOS
  • iNOS
  • iNos
  • NOS2a
  • NOS2
  • BmNOS2
  • Nsl
  • nitric oxide synthase 2
  • nitric oxide synthase 2, inducible
  • inducible nitric oxide synthase
  • NOS2
  • Nos2
  • nos2
Epitop
C-Term
32
31
16
15
9
8
7
7
7
7
6
5
5
5
5
5
4
4
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Reaktivität
Human
250
124
95
17
2
2
Wirt
Kaninchen
257
46
1
Klonalität
Polyklonal
Konjugat
Dieser NOS2 Antikörper ist unkonjugiert
17
10
5
5
5
5
5
5
4
4
3
3
2
2
1
1
1
1
1
Applikation
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
217
130
107
58
36
31
28
20
19
19
13
9
1
Optionen
Immunogen A synthetic peptide corresponding to a sequence at the C-terminal of human NOS2, identical to the related rat and mouse sequences.< br/>Immunogen was affinity purified.
Isotyp IgG
Kreuzreaktivität Maus, Ratte (Rattus)
Kreuzreaktivität (Details) No cross reactivity with other proteins.
Produktmerkmale Rabbit IgG polyclonal antibody for Nitric oxide synthase, inducible (NOS2) detection. Tested with WB, IHC-P in Human, Mouse, Rat.
Plasmids, Primers & others Plasmide, Primers & weitere NOS2 products on genomics-online (e.g. as negative or positive controls)
Antigen
Andere Bezeichnung Nitric oxide synthase, inducible (NOS2 Antibody Abstract)
Hintergrund Nitric oxide synthase, inducible is an enzyme that in humans is encoded by the NOS2 gene. Nitric oxide (NO) is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter, it is implicated in neurotoxicity associated with stroke and neurodegenerative diseases, neural regulation of smooth muscle, including peristalsis, and penile erection. Three different NOS isoforms have been identified which fall into two distinct types, constitutive and inducible. The inducible NOS (iNOS) isoform is expressed in a variety of cell types and tissues in response to inflammatory agents and cytokines. The human iNOS (NOS2) gene is approximately 37 kb in length and consists of 26 exons and 25 introns. Diefenbach et al. (1999) studied the relationship of IL12 and nitric oxide synthase-2 (NOS2) to innate immunity to the parasite Leishmania in mice. And conclude that NOS2-derived NO is a prerequisite for cytokine signaling and function in innate immunity. From studies in Tanzania and Kenya, Hobbs et al. (2002) identified a novel single-nucleotide polymorphism, -1173C-T (163730. 0001), in the NOS2 promoter that was significantly associated with protection from symptomatic malaria and severe malarial anemia.
Pathways Retinoic Acid Receptor Signaling Pathway, Cellular Response to Molecule of Bacterial Origin, Inositol Metabolic Process, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Lyophilized
Rekonstitution Add 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Buffer Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
Konservierungs-mittel Sodium azide, Thimerosal (Merthiolate)
Handhabung Avoid repeated freezing and thawing.
Lagerung -20 °C
Informationen zur Lagerung At -20 °C for one year. After reconstitution, at 4 °C for one month. It can also be aliquotted and stored frozen at -20 °C for a longer time.
Produkt verwendet in: Hobbs, Udhayakumar, Levesque, Booth, Roberts, Tkachuk, Pole, Coon, Kariuki, Nahlen, Mwaikambo, Lal, Granger, Anstey, Weinberg: "A new NOS2 promoter polymorphism associated with increased nitric oxide production and protection from severe malaria in Tanzanian and Kenyan children." in: Lancet (London, England), Vol. 360, Issue 9344, pp. 1468-75, 2002 (PubMed).

Diefenbach, Schindler, Röllinghoff, Yokoyama, Bogdan: "Requirement for type 2 NO synthase for IL-12 signaling in innate immunity." in: Science (New York, N.Y.), Vol. 284, Issue 5416, pp. 951-5, 1999 (PubMed).

Chartrain, Geller, Koty, Sitrin, Nussler, Hoffman, Billiar, Hutchinson, Mudgett: "Molecular cloning, structure, and chromosomal localization of the human inducible nitric oxide synthase gene." in: The Journal of biological chemistry, Vol. 269, Issue 9, pp. 6765-72, 1994 (PubMed).

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