NOS2 Antikörper (C-Term)

Produktnummer ABIN966678

Produktdetails anti-NOS2 Antikörper

Target: NOS2 (Nitric Oxide Synthase 2, Inducible (NOS2))

Bindungsspezifität: C-Term

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NOS2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Kreuzreaktivität: Maus, Ratte (Rattus)

Kreuzreaktivität (Details): No cross reactivity with other proteins.

Produktmerkmale: Rabbit IgG polyclonal antibody for Nitric oxide synthase, inducible (NOS2) detection. Tested with WB, IHC-P in Human, Mouse, Rat.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminal of human NOS2, identical to the related rat and mouse sequences.< br/>Immunogen was affinity purified.

Isotyp: IgG

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 µg/mL.

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide, Thimerosal (Merthiolate)

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: At -20 °C for one year. After reconstitution, at 4 °C for one month. It can also be aliquotted and stored frozen at -20 °C for a longer time.

Referenzen für anti-NOS2 Antikörper (ABIN966678)

Hobbs, Udhayakumar, Levesque, Booth, Roberts, Tkachuk, Pole, Coon, Kariuki, Nahlen, Mwaikambo, Lal, Granger, Anstey, Weinberg: "A new NOS2 promoter polymorphism associated with increased nitric oxide production and protection from severe malaria in Tanzanian and Kenyan children." in: Lancet (London, England), Vol. 360, Issue 9344, pp. 1468-75, (2002) (PubMed).

Diefenbach, Schindler, Röllinghoff, Yokoyama, Bogdan: "Requirement for type 2 NO synthase for IL-12 signaling in innate immunity." in: Science (New York, N.Y.), Vol. 284, Issue 5416, pp. 951-5, (1999) (PubMed).

Chartrain, Geller, Koty, Sitrin, Nussler, Hoffman, Billiar, Hutchinson, Mudgett: "Molecular cloning, structure, and chromosomal localization of the human inducible nitric oxide synthase gene." in: The Journal of biological chemistry, Vol. 269, Issue 9, pp. 6765-72, (1994) (PubMed).

Details zu NOS2

Target: NOS2 (Nitric Oxide Synthase 2, Inducible (NOS2))

Andere Bezeichnung: Nitric oxide synthase, inducible (NOS2 Produkte)

Synonyme: HEP-NOS antikoerper, INOS antikoerper, NOS antikoerper, NOS2A antikoerper, NOS-II antikoerper, Nos-2 antikoerper, Nos2a antikoerper, i-NOS antikoerper, iNOS antikoerper, iNos antikoerper, NOS2a antikoerper, NOS2 antikoerper, BmNOS2 antikoerper, Nsl antikoerper, nos2 antikoerper, nitric oxide synthase 2 antikoerper, nitric oxide synthase 2, inducible antikoerper, inducible nitric oxide synthase antikoerper, inducible nitric-oxide synthase antikoerper, nanos C2HC-type zinc finger 2 antikoerper, NOS2 antikoerper, Nos2 antikoerper, nos2 antikoerper, LOC396821 antikoerper, NANOS2 antikoerper

Hintergrund: Nitric oxide synthase, inducible is an enzyme that in humans is encoded by the NOS2 gene. Nitric oxide (NO) is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter, it is implicated in neurotoxicity associated with stroke and neurodegenerative diseases, neural regulation of smooth muscle, including peristalsis, and penile erection. Three different NOS isoforms have been identified which fall into two distinct types, constitutive and inducible. The inducible NOS (iNOS) isoform is expressed in a variety of cell types and tissues in response to inflammatory agents and cytokines. The human iNOS (NOS2) gene is approximately 37 kb in length and consists of 26 exons and 25 introns. Diefenbach et al. (1999) studied the relationship of IL12 and nitric oxide synthase-2 (NOS2) to innate immunity to the parasite Leishmania in mice. And conclude that NOS2-derived NO is a prerequisite for cytokine signaling and function in innate immunity. From studies in Tanzania and Kenya, Hobbs et al. (2002) identified a novel single-nucleotide polymorphism, -1173C-T (163730. 0001), in the NOS2 promoter that was significantly associated with protection from symptomatic malaria and severe malarial anemia.

Pathways: Retinoic Acid Receptor Signaling Pathway, Cellular Response to Molecule of Bacterial Origin, Inositol Metabolic Process, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process