Acetylcholinesterase Antikörper

Produktnummer ABIN1607621

Produktdetails anti-Acetylcholinesterase Antikörper

Target: Acetylcholinesterase (AChE)

Reaktivität: Rind (Kuh)

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Acetylcholinesterase Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Kreuzreaktivität (Details): Cross reactivity against Acetyl Cholinesterase from other sources is unknown.

Reinheit: Anti-ACETYL CHOLINESTERASE is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Acetyl Cholinesterase [Bovine Erythrocytes].

Endotoxin-Niveau: Low Endotoxin : No

Immunogen: Acetyl Cholinesterase [Bovine Erythrocytes]
Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ACETYL CHOLINESTERASE Antibody has been assayed against 1.0 µg of Acetyl Cholinesterase [Bovine Erythrocytes] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) code #605-4302 and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:1.200 to 1:5.000 of the reconstitution concentration is suggested for this product.
ELISA Dilution: 1:5.000 - 1:20.000
IF Immunoprecipitation Dilution: 1:100
Western Blot Dilution: 1:500 - 1:5.000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Handhabung: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Haltbarkeit: Expiration date is one (1) year from date of opening.

Details zu Acetylcholinesterase

Target: Acetylcholinesterase (AChE)

Andere Bezeichnung: Acetyl Cholinesterase (AChE Produkte)

Synonyme: ACEE antikoerper, ARACHE antikoerper, N-ACHE antikoerper, YT antikoerper, Ache antikoerper, GB14873 antikoerper, zgc:92550 antikoerper, ACE antikoerper, Dsim\\GD20515 antikoerper, GD20515 antikoerper, dsim_GLEANR_4292 antikoerper, ache antikoerper, mE1a antikoerper, mE1b antikoerper, mE1c antikoerper, mE1c-long antikoerper, mE1d antikoerper, mE1d' antikoerper, mE1e antikoerper, arache antikoerper, n-ache antikoerper, acetylcholinesterase (Cartwright blood group) antikoerper, acetylcholinesterase 2 antikoerper, acetylcholinesterase antikoerper, Acetylcholine esterase antikoerper, acetylcholinesterase (Cartwright blood group) L homeolog antikoerper, collagen type I alpha 2 chain antikoerper, ACHE antikoerper, AChE-2 antikoerper, Ache antikoerper, ache antikoerper, Dsim\Ace antikoerper, ache.L antikoerper, COL1A2 antikoerper, ACE-1 antikoerper

Hintergrund: Synonyms: Acetylcholinesterase, AChE

Gen-ID: 540446

UniProt: P23795

Pathways: Skeletal Muscle Fiber Development