T7-Tag Antikörper
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- Target Alle T7-Tag Produkte
- T7-Tag
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Wirt
- Kaninchen
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Klonalität
- Polyklonal
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Konjugat
- Dieser T7-Tag Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), ELISA
- Sequenz
- MASMTGGQQM G
- Aufreinigung
- Affinity purified
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anti-T7-Tag antibody
WB Wirt: Maus Monoclonal 1M4 unconjugated
anti-T7-Tag antibodyWB Wirt: Maus Monoclonal 1H5 unconjugated
anti-T7-Tag antibodyWB Wirt: Ziege Polyclonal unconjugated
anti-T7-Tag antibodyWB, IP Wirt: Maus Monoclonal unconjugated
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- Applikationshinweise
- Western blot: Use at 1:5,000 - 1:10,000
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Buffer
- PBS, pH 7.2 and 0.1% sodium azide.
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- 4 °C
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- Target
- T7-Tag
- Andere Bezeichnung
- T7 tag (T7-Tag Produkte)
- Substanzklasse
- Tag
- Hintergrund
- Rabbits were immunized with MASMTGGQQMG (T7 epitope) conjugated to KLH. After multiple immunizations in Freund's adjuvant, serum was collected. Antibodies were immunoaffinity-purified using the peptide conjugated to a solid-phase support. Specification: The affinity-purified antibodies were analyzed using ELISA and Western blot analyses. The anti-T7 diluted 1:35,000 gave an OD = 1.0 in a 15 minute reaction against peptide conjugated with a different carrier than was used for purification. HRP-conjugated donkey anti-rabbit IgG was used and TMB was the substrate.
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