Specific for the Rubella E1 protein and reacts with non-reduced antigens on Western blots. Exhibits a low level of HI activity. Exhibits neutralizing activity.
Reaktivität: Rubella Virus (RuV), Virus
IF (cc), IF (p)
Wirt: Kaninchen
Polyclonal
AbBy Fluor® 750
Applikationshinweise
Suitable for use in ELISA and Western blot (non-reducing conditions). Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded. Direct ELISA Antigen: Dilution series of Recombinant Rubella Virus E1 Protein, coated at 2.5 – 0.15ug/well Antibody: HRP-labeled MAb C66503M at 1.25ug/well, incubated 60 minutes at room temp., followed by TMB substrate
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Buffer
PBS, pH 7.4 + 0.2
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
Avoid multiple freeze/thaw cycles. Centrifuge product if not completely clear after standing at room temperature. Prepare working dilution only prior to immediate use.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Short term (up to 7 days) store at 2-8 °C. Long term, aliquot and store at -28 °C.