Ataxin 1 Antikörper (AA 686-699)

Produktnummer ABIN793324

Produktdetails anti-Ataxin 1 Antikörper

Target: Ataxin 1 (ATXN1)

Bindungsspezifität: AA 686-699

Reaktivität: Human, Maus, Ratte, Affe, Rind (Kuh), Schwein, Pferd, Fledermaus, Hamster

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Ataxin 1 Antikörper ist unkonjugiert

Applikation: ELISA

Spezifität: Human ATXN1 / Ataxin-1. Reported variants represent identical protein: NP_001121636.1, NP_000323.2

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Bovine, Bat, Horse, Pig, Opossum (100%) Turkey, Chicken, Lizard, Xenopus (93%) Panda, Dog, Platypus, Pufferfish (86%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide C-TLKNLKNGSVKKGQ from an internal region of human ATXN1 / Ataxin-1 (NP_000323.2). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Bovine, Bat, Horse, Pig, Opossum (100%), Turkey, Chicken, Lizard, Xenopus (93%), Panda, Dog, Platypus, Pufferfish (86%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: Peptide-ELISA (1:32000)

Usage: Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Preliminary experiments gave bands at approx 75kD and 26kD in Mouse Fetal Brain lysates after 0.5 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 84.7kD according to NP_001186234.1 and 83.8kD according to NP_033150.2. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).

Not recommended for: IHC-P

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Avoid freeze-thaw cycles.

Details zu Ataxin 1

Target: Ataxin 1 (ATXN1)

Andere Bezeichnung: ATXN1 / Ataxin-1 / SCA1 (ATXN1 Produkte)

Synonyme: ATX1 antikoerper, D6S504E antikoerper, SCA1 antikoerper, ATXN1 antikoerper, ataxin 1b antikoerper, atxn1 antikoerper, 2900016G23Rik antikoerper, Atx1 antikoerper, C85907 antikoerper, ENSMUSG00000074917 antikoerper, Gm10786 antikoerper, Sca1 antikoerper, CG4547 antikoerper, Dmel\\CG4547 antikoerper, dAtx-1 antikoerper, dAtx1 antikoerper, sca1 antikoerper, ataxin 1 antikoerper, ataxin 1b antikoerper, Ataxin 1 antikoerper, ATXN1 antikoerper, atxn1b antikoerper, Atxn1 antikoerper, Atx-1 antikoerper

Hintergrund: Name/Gene ID: ATXN1

Synonyms: ATXN1, ATX1, Ataxin 1, D6S504E, Ataxin-1, SCA1

Gen-ID: 6310

NCBI Accession: NP_000323

UniProt: P54253

Pathways: Synaptic Membrane