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Rekombinanter MTOR Antikörper (pSer2448)

Name: Rekombinanter MTOR Antikörper (pSer2448)
Brand: antibodies-online
SKU: ABIN7799348
Price: 598.92 EUR
Availability: InStock

Dieser Kaninchen Monoklonal Antikörper erkennt spezifisch MTOR in WB, FACS, ICC und IF (p). Er zeigt eine Reaktivität gegenüber Human.

Western blotting analysis using Phospho-mTOR (S(ABIN7800001) antibody (ABIN7799348). (Rekombinanter MTOR Antikörper (pSer2448))

Immunohistochemistry was performed on paraffin-embedded human breast carcinoma using phospho-mTOR (S(ABIN7800001) antibody (ABIN7799348), 1:200). (Rekombinanter MTOR Antikörper (pSer2448))

IHC (p)

Flow cytometric analysis of Phospho-mTOR (S(ABIN7800001) expression in HeLa cells using Phospho-mTOR (S(ABIN7800001) antibody (ABIN7799348), 1:2,000). (Rekombinanter MTOR Antikörper (pSer2448))

FACS

Validation of mTOR knockdown using flow cytometry. (Rekombinanter MTOR Antikörper (pSer2448))

FACS

Immunocytochemical staining of HeLa cells with Phospho-mTOR (S(ABIN7800001) antibody (ABIN7799348), 1:1,000). (Rekombinanter MTOR Antikörper (pSer2448))

ICC

Immunocytochemical staining of HeLa cells using Phospho-mTOR (S(ABIN7800001) antibody (ABIN7799348), 1:1,000), Top panel: wild-type (WT), Bottom panal: Phospho-mTOR (S(ABIN7800001) shRNA knockdown (KD). (Rekombinanter MTOR Antikörper (pSer2448))

ICC

7 Abbildungen

Produktnummer ABIN7799348

Kurzübersicht für Rekombinanter MTOR Antikörper (pSer2448) (ABIN7799348)

Target

Alle MTOR (mTOR) Antikörper anzeigen

MTOR (mTOR) (Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR))

Antikörpertyp

Recombinant Antibody

Reaktivität

Human

Wirt

Kaninchen

Klonalität

Monoklonal

Konjugat

Dieser MTOR Antikörper ist unkonjugiert

Applikation

Western Blotting (WB), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Güteklasse

KD Validated

Klon

24GB330

Discover our top product mTOR Primärantikörper

Bindungsspezifität
37

36

21

15

11

10

5

5

4

4

4

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
pSer2448

Expressionssystem

HEK-293 Cells

Verwendungszweck

KD-Validated Phospho-mTOR (S2448) Recombinant Rabbit mAb

Kreuzreaktivität

Maus, Ratte

Aufreinigung

Affinity Purified

Immunogen

A synthesized peptide derived from human Phospho-mTOR (S2448)

Isotyp

IgG
anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR) (AA 2436-2492) antibody
mTOR Reaktivität: Human, Maus, Ratte WB, ELISA, FACS, IHC (p), IF (p), IF (cc), IHC (fro) Wirt: Kaninchen Polyclonal unconjugated

anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR) (C-Term) antibody
mTOR Reaktivität: Human, Maus, Ratte WB, IF, IP, ICC, IHC (p), ChIP Wirt: Kaninchen Polyclonal unconjugated

anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR) (AA 2226-2488) antibody
mTOR Reaktivität: Human WB, IHC, IP, ICC Wirt: Kaninchen Polyclonal unconjugated

anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR) (AA 2400-2500) antibody
mTOR Reaktivität: Human WB, IF Wirt: Kaninchen Polyclonal unconjugated

anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR) (AA 2459-2488) antibody
mTOR Reaktivität: Human WB, IF, IHC (p) Wirt: Kaninchen Polyclonal RB13347 unconjugated

anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR) antibody
mTOR Reaktivität: Human, Maus, Ratte, Fisch WB, IHC, ELISA, IF, ICC Wirt: Kaninchen Polyclonal unconjugated

anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR) (AA 2311-2529) antibody
mTOR Reaktivität: Human IHC, ELISA Wirt: Maus Monoclonal 8H5A5 unconjugated

anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR) antibody
KD Validated mTOR Reaktivität: Human WB, FACS, ICC Wirt: Kaninchen Monoclonal 24GB200 unconjugated Recombinant Antibody

anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR) (AA 2226-2488) antibody
mTOR Reaktivität: Human WB, IHC Wirt: Maus Monoclonal D7 unconjugated

anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR) (pSer2481) antibody
mTOR Reaktivität: Human, Maus, Ratte WB, ELISA, FACS, IHC (p), IF (p), IF (cc), IHC (fro) Wirt: Kaninchen Polyclonal unconjugated

Applikationshinweise

Western Blotting (WB): 1:1,000-1:5,000, Flow Cytometry (FCM): 1:200-1:2,000, Immunocytochemistry (ICC): 1:100-1:1,000, Immunohistochemistry-Paraffin (IHC-P): 1:100-1:200

Kommentare

We assess the antibodies’ specificity and selectivity using knockdown techniques. Our validation approach is straightforward: if an antibody identifies a signal in wild-type (WT) cells, but this signal is absent or noticeably reduced in gene-silenced (KD) cells, we consider the antibody to be highly specific.

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Liquid

Buffer

PBS, pH 7.4, with 0.02 % sodium azide

Konservierungsmittel

Sodium azide

Vorsichtsmaßnahmen

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung

-20 °C

Informationen zur Lagerung

Store at -20 °C for one year.

Haltbarkeit

12 months
Target

MTOR (mTOR) (Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR))

Andere Bezeichnung

mTOR

Molekulargewicht

289 kDa

Gen-ID

2475

UniProt

P42345

Pathways

PI3K-Akt Signalweg, RTK Signalweg, AMPK Signaling, Interferon-gamma Pathway, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Regulation of Actin Filament Polymerization, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Autophagie, CXCR4-mediated Signaling Events, BCR Signaling, Warburg Effekt