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RAF1 Antikörper (pSer338, pTyr340)

Der Kaninchen Polyklonal Anti-RAF1-Antikörper wurde für WB, ELISA und IHC (p) validiert. Er ist geeignet, RAF1 in Proben von Maus und Ratte zu detektieren.
Produktnummer ABIN733628

Kurzübersicht für RAF1 Antikörper (pSer338, pTyr340) (ABIN733628)

Target

Alle RAF1 Antikörper anzeigen
RAF1 (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1))

Reaktivität

  • 439
  • 209
  • 139
  • 14
  • 12
  • 12
  • 11
  • 9
  • 5
  • 4
  • 4
  • 2
  • 2
  • 2
  • 2
  • 1
Maus, Ratte

Wirt

  • 471
  • 15
  • 2
  • 2
Kaninchen

Klonalität

  • 438
  • 52
Polyklonal

Konjugat

  • 209
  • 33
  • 27
  • 26
  • 16
  • 15
  • 10
  • 10
  • 10
  • 10
  • 10
  • 10
  • 10
  • 10
  • 10
  • 10
  • 10
  • 9
  • 9
  • 9
  • 9
  • 9
  • 9
Dieser RAF1 Antikörper ist unkonjugiert

Applikation

  • 299
  • 207
  • 104
  • 104
  • 95
  • 73
  • 43
  • 35
  • 28
  • 24
  • 17
  • 16
  • 16
  • 3
  • 2
  • 2
  • 1
Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
  • Bindungsspezifität

    • 48
    • 48
    • 35
    • 33
    • 27
    • 24
    • 24
    • 23
    • 23
    • 20
    • 20
    • 15
    • 15
    • 14
    • 12
    • 8
    • 8
    • 8
    • 8
    • 6
    • 6
    • 6
    • 5
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    pSer338, pTyr340

    Spezifität

    These phosphorylation sites are homologous across the species listed.

    Kreuzreaktivität

    Maus, Ratte

    Homologie

    Human,Dog,Cow,Pig,Horse,Chicken,Rabbit,Guinea Pig

    Aufreinigung

    Purified by Protein A.

    Immunogen

    KLH conjugated synthetic phosphopeptide derived from human c-Raf around the phosphorylation site of Ser338/Tyr340

    Isotyp

    IgG
  • Applikationshinweise

    WB 1:300-5000
    ELISA 1:500-1000
    IHC-P 1:200-400

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Liquid

    Konzentration

    1 μg/μL

    Buffer

    0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

    Konservierungsmittel

    ProClin

    Vorsichtsmaßnahmen

    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

    Haltbarkeit

    12 months
  • Target

    RAF1 (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1))

    Andere Bezeichnung

    c-Raf

    Hintergrund

    Synonyms: NS5, CRAF, Raf-1, c-Raf, CMD1NN, RAF proto-oncogene serine/threonine-protein kinase, Proto-oncogene c-RAF, RAF1, RAF

    Background: Serine/threonine-protein kinase that acts as a regulatory link between the membrane-associated Ras GTPases and the MAPK/ERK cascade, and this critical regulatory link functions as a switch determining cell fate decisions including proliferation, differentiation, apoptosis, survival and oncogenic transformation. RAF1 activation initiates a mitogen-activated protein kinase (MAPK) cascade that comprises a sequential phosphorylation of the dual-specific MAPK kinases (MAP2K1/MEK1 and MAP2K2/MEK2) and the extracellular signal-regulated kinases (MAPK3/ERK1 and MAPK1/ERK2). The phosphorylated form of RAF1 (on residues Ser-338 and Ser-339, by PAK1) phosphorylates BAD/Bcl2-antagonist of cell death at 'Ser-75'. Phosphorylates adenylyl cyclases: ADCY2, ADCY5 and ADCY6, resulting in their activation. Phosphorylates PPP1R12A resulting in inhibition of the phosphatase activity. Phosphorylates TNNT2/cardiac muscle troponin T. Can promote NF-kB activation and inhibit signal transducers involved in motility (ROCK2), apoptosis (MAP3K5/ASK1 and STK3/MST2), proliferation and angiogenesis (RB1). Can protect cells from apoptosis also by translocating to the mitochondria where it binds BCL2 and displaces BAD/Bcl2-antagonist of cell death. Regulates Rho signaling and migration, and is required for normal wound healing. Plays a role in the oncogenic transformation of epithelial cells via repression of the TJ protein, occludin (OCLN) by inducing the up-regulation of a transcriptional repressor SNAI2/SLUG, which induces down-regulation of OCLN. Restricts caspase activation in response to selected stimuli, notably Fas stimulation, pathogen-mediated macrophage apoptosis, and erythroid differentiation.

    Gen-ID

    5894

    UniProt

    P04049

    Pathways

    MAPK Signalweg, RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, Neurotrophin Signalübertragung, cAMP Metabolic Process, Stem Cell Maintenance, Hepatitis C, Autophagie, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling, BCR Signaling
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