BMI1 Proto-Oncogene, Polycomb Ring Finger (BMI1) Antikörper

Produktnummer ABIN7468056

Produktdetails

Target: BMI1 Proto-Oncogene, Polycomb Ring Finger (BMI1)

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Applikation: Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Kreuzreaktivität: Cat, Hund, Human, Maus

Aufreinigung: Purified by antigen-affinity chromatography.

Güteklasse: KO Validated

Immunogen: Recombinant protein encompassing a sequence within the center region of human Bmi1. The exact sequence is proprietary.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Kommentare:

Positive Control: THP-1 , HL-60 , C8D30 , BCL-1 , Raw264.7 , K562 , THP-1 , 293T , A549 , H1299 , HCT116 Validation: KO/KD, Orthogonal

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 20 % Glycerol, 0.01 % Thimerosal

Konservierungsmittel: Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Antigendetails

Target: BMI1 Proto-Oncogene, Polycomb Ring Finger (BMI1)

Andere Bezeichnung: BMI1 proto-oncogene, polycomb ring finger

Synonyme: FLVI2/BMI1 antikoerper, PCGF4 antikoerper, RNF51 antikoerper, BMI1 proto-oncogene, polycomb ring finger antikoerper, BMI1 antikoerper

Hintergrund:

Synonyms: BMI1 proto-oncogene, polycomb ring finger , FLVI2/BMI1 , PCGF4 , RNF51 , flvi-2/bmi-1

Background: Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones, it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2.

Molekulargewicht: 37 kDa

Gen-ID: 648

UniProt: P35226