PKC alpha Antikörper (AA 622-672)

Produktnummer ABIN7453397

Der Kaninchen Polyklonal anti-PKC alpha Antikörper wird verwendet zum Nachweis von PKC alpha in Proben von Human und Maus. Er wurde validiert für WB, IP und IF (p).

Kurzübersicht für PKC alpha Antikörper (AA 622-672) (ABIN7453397)

Target: PKC alpha (PKCa) (Protein Kinase C, alpha (PKCa))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PKC alpha Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Produktdetails anti-PKC alpha Antikörper

Bindungsspezifität: AA 622-672

Verwendungszweck: Rabbit anti-PKC-alpha Antibody, Affinity Purified

Homologie: Rat,Bovine

Aufreinigung: Affinity Purified

Immunogen: between AA 622 and 672

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

IHC-IF: 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 5 μg/mg lysate

WB: 1:2,000 - 1:10,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: Tris-buffered Saline containing 0.1 % BSA and 0.09 % Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Haltbarkeit: 12 months

Details zu PKC alpha

Target: PKC alpha (PKCa) (Protein Kinase C, alpha (PKCa))

Andere Bezeichnung: PKC-alpha

Hintergrund: Background: Protein kinase C alpha (PKC-alpha) is a member of the PKC family of serine-threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. PKC-alpha has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control [taken from NCBI Entrez Gene (GeneID: 5578)].

Gen-ID: 5578

NCBI Accession: NP_002728

UniProt: P17252

Pathways: WNT Signalweg, T-Zell Rezeptor Signalweg, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Thyroid Hormone Synthesis, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Regulation of G-Protein Coupled Receptor Protein Signaling, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling