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PKC alpha Antikörper (N-Term)

Der Kaninchen Polyklonal anti-PKC alpha Antikörper wird verwendet zum Nachweis von PKC alpha in Proben von Human und Maus. Er wurde validiert für WB, IF, FACS und IHC (p).

Western blot analysis of PRKCA Antibody (N-term) (ABIN652517 and ABIN2842346) in mouse NIH-3T3 cell line lysates (35 μg/lane). (PKC alpha Antikörper (N-Term))

PRKCA Antibody (N-term) (ABIN652517 and ABIN2842346) flow cytometric analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram). (PKC alpha Antikörper (N-Term))

FACS

Confocal immunofluorescent analysis of PRKCA Antibody (N-term) (ABIN652517 and ABIN2842346) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). (PKC alpha Antikörper (N-Term))

Formalin-fixed and paraffin-embedded human lung carcinoma reacted with PRKCA Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. (PKC alpha Antikörper (N-Term))

IHC (p)

4 Abbildungen

Produktnummer ABIN652517

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Kurzübersicht für PKC alpha Antikörper (N-Term) (ABIN652517)

Target

Alle PKC alpha (PKCa) Antikörper anzeigen

PKC alpha (PKCa) (Protein Kinase C, alpha (PKCa))

Reaktivität

Human, Maus

Wirt

Kaninchen

Klonalität

Polyklonal

Konjugat

Dieser PKC alpha Antikörper ist unkonjugiert

Applikation

Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon

RB20769

Discover our top product PKCa Primärantikörper

Bindungsspezifität
50

24

18

15

14

13

12

10

8

8

6

5

4

4

3

3

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 75-103, N-Term

Homologie

B, Rb, Rat

Aufreinigung

This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen

This PRKCA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 75-103 amino acids from the N-terminal region of human PRKCA.

Isotyp

Ig Fraction
anti-Protein Kinase C, alpha (PKCa) antibody
KD Validated PKCa Reaktivität: Human WB, ICC, FACS, IF (p) Wirt: Kaninchen Monoclonal 23GB1690 unconjugated Recombinant Antibody

anti-Protein Kinase C, alpha (PKCa) (pThr497) antibody
KD Validated PKCa Reaktivität: Human WB, FACS, IF (p) Wirt: Kaninchen Monoclonal 23GB4955 unconjugated Recombinant Antibody

anti-Protein Kinase C, alpha (PKCa) antibody
KD Validated PKCa Reaktivität: Human WB, FACS, IF (p) Wirt: Kaninchen Monoclonal 24GB4475 unconjugated Recombinant Antibody

anti-Protein Kinase C, alpha (PKCa) (AA 563-672) antibody
PKCa Reaktivität: Human WB, ELISA, IF, IHC (p), PLA Wirt: Maus Monoclonal 2F11 unconjugated

anti-Protein Kinase C, alpha (PKCa) (AA 299-329), (C-Term) antibody
PKCa Reaktivität: Human WB, IF, FACS, IHC (p) Wirt: Kaninchen Polyclonal RB01012 unconjugated

anti-Protein Kinase C, alpha (PKCa) (AA 153-342) antibody
PKCa Reaktivität: Human, Maus, Ratte WB, IHC Wirt: Kaninchen Polyclonal unconjugated

anti-Protein Kinase C, alpha (PKCa) (pSer657) antibody
KD Validated PKCa Reaktivität: Human WB, FACS Wirt: Kaninchen Monoclonal 23GB1055 unconjugated Recombinant Antibody

anti-Protein Kinase C, alpha (PKCa) (pThr638) antibody
PKCa Reaktivität: Human WB, IF, ICC, FACS, IHC (p), IHC (fro) Wirt: Kaninchen Monoclonal unconjugated Recombinant Antibody

anti-Protein Kinase C, alpha (PKCa) (AA 299-329) antibody
PKCa Reaktivität: Human WB, IHC, ELISA, IF, FACS Wirt: Kaninchen Polyclonal unconjugated

anti-Protein Kinase C, alpha (PKCa) antibody
PKCa Reaktivität: Human WB, IHC, IP Wirt: Kaninchen Monoclonal unconjugated

Applikationshinweise

IF: 1:10~50. WB: 1:1000. IHC-P: 1:50~100. FC: 1:10~50

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Liquid

Buffer

Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Konservierungsmittel

Sodium azide

Vorsichtsmaßnahmen

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung

4 °C,-20 °C

Informationen zur Lagerung

Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Haltbarkeit

6 months
Target

PKC alpha (PKCa) (Protein Kinase C, alpha (PKCa))

Andere Bezeichnung

PRKCA

Hintergrund

PRKCA is a calcium-activated, phospholipid-dependent, serine-and threonine-specific enzyme. It may play a role in cell motility by phosphorylating CSPG4. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.

Molekulargewicht

76750

Gen-ID

5578

NCBI Accession

NP_002728

UniProt

P17252

Pathways

WNT Signalweg, T-Zell Rezeptor Signalweg, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Thyroid Hormone Synthesis, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Regulation of G-Protein Coupled Receptor Protein Signaling, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling