Rekombinanter PKC alpha Antikörper

Produktnummer ABIN7799989

Der Kaninchen Monoklonal anti-PKC alpha Antikörper (Klon 23GB1690) (ABIN7799989) detektiert spezifisch PKC alpha in WB, ICC, FACS und IF (p). Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für Rekombinanter PKC alpha Antikörper (ABIN7799989)

Target: PKC alpha (PKCa) (Protein Kinase C, alpha (PKCa))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser PKC alpha Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Güteklasse: KD Validated

Klon: 23GB1690

Produktdetails anti-PKC alpha Antikörper

Expressionssystem: HEK-293 Cells

Verwendungszweck: KD-Validated PKC alpha Recombinant Rabbit mAb

Kreuzreaktivität: Maus, Ratte

Aufreinigung: Affinity Purified

Immunogen: A synthesized peptide derived from human PKC alpha

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western Blotting (WB): 1:1,000-1:5,000, Flow Cytometry (FCM): 1:200-1:2,000, Immunocytochemistry (ICC): 1:100-1:1,000, Immunohistochemistry-Paraffin (IHC-P): 1:100-1:200

Kommentare:

We assess the antibodies’ specificity and selectivity using knockdown techniques. Our validation approach is straightforward: if an antibody identifies a signal in wild-type (WT) cells, but this signal is absent or noticeably reduced in gene-silenced (KD) cells, we consider the antibody to be highly specific.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: PBS (pH 7.4) containing 50% glycerol and 0.02% sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20 °C for one year.

Haltbarkeit: 12 months

Details zu PKC alpha

Target: PKC alpha (PKCa) (Protein Kinase C, alpha (PKCa))

Andere Bezeichnung: PKC alpha

Molekulargewicht: 77 kDa

Gen-ID: 5578

UniProt: P17252

Pathways: WNT Signalweg, T-Zell Rezeptor Signalweg, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Thyroid Hormone Synthesis, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Regulation of G-Protein Coupled Receptor Protein Signaling, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling