PKC alpha Antikörper (AA 622-672)

Produktnummer ABIN7453397

Der Kaninchen Polyklonal Anti-PKC alpha-Antikörper wurde für WB, IP und IF (p) validiert. Er ist geeignet, PKC alpha in Proben von Human und Maus zu detektieren.

Kurzübersicht für PKC alpha Antikörper (AA 622-672) (ABIN7453397)

Target: PKC alpha (PKCa) (Protein Kinase C, alpha (PKCa))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PKC alpha Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Produktdetails anti-PKC alpha Antikörper

Bindungsspezifität: AA 622-672

Verwendungszweck: Rabbit anti-PKC-alpha Antibody, Affinity Purified

Homologie: Rat,Bovine

Aufreinigung: Affinity Purified

Immunogen: between AA 622 and 672

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

IHC-IF: 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 5 μg/mg lysate

WB: 1:2,000 - 1:10,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: Tris-buffered Saline containing 0.1 % BSA and 0.09 % Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Haltbarkeit: 12 months

Details zu PKC alpha

Target: PKC alpha (PKCa) (Protein Kinase C, alpha (PKCa))

Andere Bezeichnung: PKC-alpha

Hintergrund: Background: Protein kinase C alpha (PKC-alpha) is a member of the PKC family of serine-threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. PKC-alpha has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control [taken from NCBI Entrez Gene (GeneID: 5578)].

Gen-ID: 5578

NCBI Accession: NP_002728

UniProt: P17252

Pathways: WNT Signalweg, T-Zell Rezeptor Signalweg, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Thyroid Hormone Synthesis, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Regulation of G-Protein Coupled Receptor Protein Signaling, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling