TagRFP Fluorescent Protein Antikörper

Produktnummer ABIN7273101

Produktdetails

Target: TagRFP Fluorescent Protein

Reaktivität: Discosoma

Wirt: Ziege

Klonalität: Polyklonal

Applikation: Immunoelectron Microscopy (IEM), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Kreuzreaktivität (Details): Reacts with Red Fluorescent Protein

Aufreinigung: Epitope affinity purified

Immunogen: Affinity purified recombinant fluorescent protein (HIR78_01785 from Bacillus subtillis) and produced in E. coli.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB:1:500-1:5,000, IF:1:50-1:500, IHC-P:1:50-1:500, IHC-F:1:50-1:500, IEM:1:50-1:500

Kommentare:

In lysates of transfected cells with the plasmid containing the fluorescent sequence, detects the recombinant protein by Western blot.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 3 mg/mL

Buffer: PBS, 20 % glycerol and 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20 °C for long-term storage. Store at 2-8 °C for up to one month.

Antigendetails

Target: TagRFP Fluorescent Protein

Hintergrund: TagRFP (orange fluorescent protein) is a basic (constitutively fluorescent) fluorescent protein, monomeric engineered derivate of fluorescent protein (RFP) isolated from members of the Entacmaea quadricolor. It has very low acid sensitivity. TagRFP is a ~26 kDa protein that is optimally excited at a 555 nm and has a maximum of emission at 584 nm. This fluorescent protein is used in research as a reporter to label and study the biology of the cell using whole body imaging.