RAF1 Antikörper (pSer339)
Kurzübersicht für RAF1 Antikörper (pSer339) (ABIN712736)
Target
Alle RAF1 Antikörper anzeigenReaktivität
Wirt
Klonalität
Konjugat
Applikation
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                                            Bindungsspezifität
- pSer339
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                                            Kreuzreaktivität
- Human, Maus
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                                            Homologie
- Rat,Dog,Cow,Sheep,Pig,Horse,Chicken,Rabbit
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                                            Aufreinigung
- Purified by Protein A.
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                                            Immunogen
- KLH conjugated synthetic phosphopeptide derived from human c-Raf around the phosphorylation site of Ser339
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                                            Isotyp
- IgG
 
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    - 
                                            Applikationshinweise
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                        WB 1:300-5000
 ELISA 1:500-1000
 IHC-P 1:200-400
 IHC-F 1:100-500
 IF(IHC-P) 1:50-200
 IF(IHC-F) 1:50-200
 IF(ICC) 1:50-200
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                                            Beschränkungen
- Nur für Forschungszwecke einsetzbar
 
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    - 
                                            Format
- Liquid
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                                            Konzentration
- 1 μg/μL
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                                            Buffer
- 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
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                                            Konservierungsmittel
- ProClin
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                                            Vorsichtsmaßnahmen
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
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                                            Lagerung
- 4 °C,-20 °C
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                                            Informationen zur Lagerung
- Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
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                                            Haltbarkeit
- 12 months
 
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    - 
                                            : "Polo-like kinase 1 induces epithelial-to-mesenchymal transition and promotes epithelial cell motility by activating CRAF/ERK signaling."    in: eLife, Vol. 5, (2016)         (PubMed).        
 
 
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                                            : "Polo-like kinase 1 induces epithelial-to-mesenchymal transition and promotes epithelial cell motility by activating CRAF/ERK signaling."    in: eLife, Vol. 5, (2016)         (PubMed).        
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    - RAF1 (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1))
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                                            Andere Bezeichnung
- c-Raf
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                                            Hintergrund
- 
                        Synonyms: NS5, CRAF, Raf-1, c-Raf, CMD1NN, RAF proto-oncogene serine/threonine-protein kinase, Proto-oncogene c-RAF, RAF1, RAF Background: Serine/threonine-protein kinase that acts as a regulatory link between the membrane-associated Ras GTPases and the MAPK/ERK cascade, and this critical regulatory link functions as a switch determining cell fate decisions including proliferation, differentiation, apoptosis, survival and oncogenic transformation. RAF1 activation initiates a mitogen-activated protein kinase (MAPK) cascade that comprises a sequential phosphorylation of the dual-specific MAPK kinases (MAP2K1/MEK1 and MAP2K2/MEK2) and the extracellular signal-regulated kinases (MAPK3/ERK1 and MAPK1/ERK2). The phosphorylated form of RAF1 (on residues Ser-338 and Ser-339, by PAK1) phosphorylates BAD/Bcl2-antagonist of cell death at 'Ser-75'. Phosphorylates adenylyl cyclases: ADCY2, ADCY5 and ADCY6, resulting in their activation. Phosphorylates PPP1R12A resulting in inhibition of the phosphatase activity. Phosphorylates TNNT2/cardiac muscle troponin T. Can promote NF-kB activation and inhibit signal transducers involved in motility (ROCK2), apoptosis (MAP3K5/ASK1 and STK3/MST2), proliferation and angiogenesis (RB1). Can protect cells from apoptosis also by translocating to the mitochondria where it binds BCL2 and displaces BAD/Bcl2-antagonist of cell death. Regulates Rho signaling and migration, and is required for normal wound healing. Plays a role in the oncogenic transformation of epithelial cells via repression of the TJ protein, occludin (OCLN) by inducing the up-regulation of a transcriptional repressor SNAI2/SLUG, which induces down-regulation of OCLN. Restricts caspase activation in response to selected stimuli, notably Fas stimulation, pathogen-mediated macrophage apoptosis, and erythroid differentiation. 
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                                            Gen-ID
- 5894
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                                            UniProt
- P04049
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                                            Pathways
- MAPK Signalweg, RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, Neurotrophin Signalübertragung, cAMP Metabolic Process, Stem Cell Maintenance, Hepatitis C, Autophagie, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling, BCR Signaling
 Target
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