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RAF1 Antikörper (pTyr341)

Der Kaninchen Polyklonal Anti-RAF1-Antikörper wurde für WB, ELISA, IF (cc), IF (p), IHC (p) und IHC (fro) validiert. Er ist geeignet, RAF1 in Proben von Human zu detektieren. Es ist 1 Publikation verfügbar.
Produktnummer ABIN712706

Kurzübersicht für RAF1 Antikörper (pTyr341) (ABIN712706)

Target

Alle RAF1 Antikörper anzeigen
RAF1 (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1))

Reaktivität

  • 438
  • 210
  • 140
  • 14
  • 12
  • 12
  • 11
  • 9
  • 5
  • 4
  • 4
  • 2
  • 2
  • 2
  • 2
  • 1
Human

Wirt

  • 471
  • 15
  • 2
  • 2
Kaninchen

Klonalität

  • 438
  • 52
Polyklonal

Konjugat

  • 209
  • 33
  • 27
  • 26
  • 16
  • 15
  • 10
  • 10
  • 10
  • 10
  • 10
  • 10
  • 10
  • 10
  • 10
  • 10
  • 10
  • 9
  • 9
  • 9
  • 9
  • 9
  • 9
Dieser RAF1 Antikörper ist unkonjugiert

Applikation

  • 299
  • 207
  • 103
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  • 16
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  • 2
  • 1
Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
  • Bindungsspezifität

    • 49
    • 48
    • 35
    • 32
    • 27
    • 24
    • 24
    • 24
    • 23
    • 20
    • 20
    • 15
    • 15
    • 14
    • 12
    • 8
    • 8
    • 8
    • 8
    • 6
    • 6
    • 6
    • 5
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
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    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    pTyr341

    Kreuzreaktivität

    Human

    Homologie

    Mouse,Rat,Dog,Cow,Pig,Horse,Chicken,Rabbit

    Aufreinigung

    Purified by Protein A.

    Immunogen

    KLH conjugated synthetic phosphopeptide derived from human c-Raf around the phosphorylation site of Tyr341

    Isotyp

    IgG
  • Applikationshinweise

    WB 1:300-5000
    ELISA 1:500-1000
    IHC-P 1:200-400
    IHC-F 1:100-500
    IF(IHC-P) 1:50-200
    IF(IHC-F) 1:50-200
    IF(ICC) 1:50-200

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Liquid

    Konzentration

    1 μg/μL

    Buffer

    0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

    Konservierungsmittel

    ProClin

    Vorsichtsmaßnahmen

    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

    Haltbarkeit

    12 months
  • Wu, Dong, Shi, Yu, Fu, Zhang, Cai, Zhao, Peng, Wu, Wu: "Regulation of integrin ?V subunit expression by sulfatide in hepatocellular carcinoma cells." in: Journal of lipid research, Vol. 54, Issue 4, pp. 936-52, (2013) (PubMed).

  • Target

    RAF1 (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1))

    Andere Bezeichnung

    c-Raf

    Hintergrund

    Synonyms: NS5, CRAF, Raf-1, c-Raf, CMD1NN, RAF proto-oncogene serine/threonine-protein kinase, Proto-oncogene c-RAF, RAF1, RAF

    Background: Serine/threonine-protein kinase that acts as a regulatory link between the membrane-associated Ras GTPases and the MAPK/ERK cascade, and this critical regulatory link functions as a switch determining cell fate decisions including proliferation, differentiation, apoptosis, survival and oncogenic transformation. RAF1 activation initiates a mitogen-activated protein kinase (MAPK) cascade that comprises a sequential phosphorylation of the dual-specific MAPK kinases (MAP2K1/MEK1 and MAP2K2/MEK2) and the extracellular signal-regulated kinases (MAPK3/ERK1 and MAPK1/ERK2). The phosphorylated form of RAF1 (on residues Ser-338 and Ser-339, by PAK1) phosphorylates BAD/Bcl2-antagonist of cell death at 'Ser-75'. Phosphorylates adenylyl cyclases: ADCY2, ADCY5 and ADCY6, resulting in their activation. Phosphorylates PPP1R12A resulting in inhibition of the phosphatase activity. Phosphorylates TNNT2/cardiac muscle troponin T. Can promote NF-kB activation and inhibit signal transducers involved in motility (ROCK2), apoptosis (MAP3K5/ASK1 and STK3/MST2), proliferation and angiogenesis (RB1). Can protect cells from apoptosis also by translocating to the mitochondria where it binds BCL2 and displaces BAD/Bcl2-antagonist of cell death. Regulates Rho signaling and migration, and is required for normal wound healing. Plays a role in the oncogenic transformation of epithelial cells via repression of the TJ protein, occludin (OCLN) by inducing the up-regulation of a transcriptional repressor SNAI2/SLUG, which induces down-regulation of OCLN. Restricts caspase activation in response to selected stimuli, notably Fas stimulation, pathogen-mediated macrophage apoptosis, and erythroid differentiation.

    Gen-ID

    5894

    UniProt

    P04049

    Pathways

    MAPK Signalweg, RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, Neurotrophin Signalübertragung, cAMP Metabolic Process, Stem Cell Maintenance, Hepatitis C, Autophagie, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling, BCR Signaling
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