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HIST1H3A Antikörper (acLys27)

Name: HIST1H3A Antikörper (acLys27)
Brand: antibodies-online
SKU: ABIN7139175
Price: 350.9 EUR
Availability: InStock

HIST1H3A Reaktivität: Human ELISA, WB, IF, ChIP, ICC, IP Wirt: Kaninchen Polyclonal unconjugated

Western Blot Detected samples: Hela whole cell lysate, MCF-7 whole cell lysate, Jurkat whole cell lysate, A549 whole cell lysate, Untreated (-) or treated (+) with 30 mM sodium butyrate for 4h All lanes: HIST1H3A antibody at 1:100 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 16 kDa Observed band size: 16 kDa (HIST1H3A Antikörper (acLys27))

Immunofluorescence staining of Hela cells (treated with 30mM sodium butyrate for 4h) with ABIN7139175 at 1:5, counter-stained with DAPI. (HIST1H3A Antikörper (acLys27))

Immunocytochemistry analysis of ABIN7139175 diluted at 1:10 and staining in Hela cells (treated with 30mM sodium butyrate for 4h) performed on a Leica BondTM system. (HIST1H3A Antikörper (acLys27))

ICC

Chromatin Immunoprecipitation Hela (4*10 6 , treated with 30 mM sodium butyrate for 4h) were treated with Benzanase, sonicated, and immunoprecipitated with 5 μg anti-HIST1H3A (ABIN7139175) or a control normal rabbit IgG. (HIST1H3A Antikörper (acLys27))

IHC

Immunoprecipitating HIST1H3A in HepG2 whole cell lysate Lane 1: Rabbit control IgG instead of ABIN7139175 in HepG2 whole cell lysate. (HIST1H3A Antikörper (acLys27))

5 Abbildungen

Produktnummer ABIN7139175

Datenblatt (PDF)

Target Alle HIST1H3A Antikörper anzeigen

HIST1H3A (Histone Cluster 1, H3a (HIST1H3A))
Bindungsspezifität
16

8

6

6

6

6

5

5

5

5

5

5

4

4

4

4

4

4

4

4

4

4

4

3

3

3

3

3

3

3

3

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2
acLys27
Reaktivität
242

67

50

16

4

4

3

2

2

2

2

2

2

1

1

1

1

1

1

1
Human
Wirt
235

6

1
Kaninchen
Passende Sekundärantikörper
Klonalität
176

66
Polyklonal
Konjugat
223

3

2

2

1

1

1

1

1

1

1

1

1

1

1

1
Dieser HIST1H3A Antikörper ist unkonjugiert
Applikation
202

182

72

68

61

57

52

27

19

17

16

13

13

7

5
ELISA, Western Blotting (WB), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Kreuzreaktivität

Human

Aufreinigung

Antigen Affinity Purified

Immunogen

Peptide sequence around site of Acetyl-Lys (27) derived from Human Histone H3.1

Isotyp

IgG

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anti-Histone Cluster 1, H3a (HIST1H3A) (3meLys9) antibody
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anti-Histone Cluster 1, H3a (HIST1H3A) (3meLys36) antibody
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anti-Histone Cluster 1, H3a (HIST1H3A) (acLys14), (acLys9) antibody
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Applikationshinweise

Recommended dilution: WB:1:100-1:1000, ICC:1:10-1:100, IF:1:1-1:10, IP:1:200-1:2000,

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Liquid

Buffer

Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, pH 7.4

Konservierungsmittel

ProClin

Vorsichtsmaßnahmen

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung

-20 °C,-80 °C

Informationen zur Lagerung

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target

HIST1H3A (Histone Cluster 1, H3a (HIST1H3A))

Andere Bezeichnung

HIST1H3A (HIST1H3A Produkte)

Synonyme

H3/A antikoerper, H3FA antikoerper, histone cluster 1 H3 family member a antikoerper, histone cluster 1, H3a antikoerper, HIST1H3A antikoerper, Hist1h3a antikoerper

Hintergrund

Background: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Aliases: H3 histone family member E pseudogene antibody, H3 histone family, member A antibody, H3/A antibody, H31_HUMAN antibody, H3F3 antibody, H3FA antibody, Hist1h3a antibody, HIST1H3B antibody, HIST1H3C antibody, HIST1H3D antibody, HIST1H3E antibody, HIST1H3F antibody, HIST1H3G antibody, HIST1H3H antibody, HIST1H3I antibody, HIST1H3J antibody, HIST3H3 antibody, histone 1, H3a antibody, Histone cluster 1, H3a antibody, Histone H3 3 pseudogene antibody, Histone H3.1 antibody, Histone H3/a antibody, Histone H3/b antibody, Histone H3/c antibody, Histone H3/d antibody, Histone H3/f antibody, Histone H3/h antibody, Histone H3/i antibody, Histone H3/j antibody, Histone H3/k antibody, Histone H3/l antibody

UniProt

P68431