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Rekombinanter RAF1 Antikörper (pSer621)

Der Kaninchen Monoklonal Anti-RAF1-Antikörper wurde für WB, ELISA und IF validiert. Er ist geeignet, RAF1 in Proben von Human zu detektieren.
Produktnummer ABIN7127739

Kurzübersicht für Rekombinanter RAF1 Antikörper (pSer621) (ABIN7127739)

Target

Alle RAF1 Antikörper anzeigen
RAF1 (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1))

Antikörpertyp

Recombinant Antibody

Reaktivität

  • 438
  • 210
  • 140
  • 14
  • 12
  • 12
  • 11
  • 9
  • 5
  • 4
  • 4
  • 2
  • 2
  • 2
  • 2
  • 1
Human

Wirt

  • 471
  • 15
  • 2
  • 2
Kaninchen

Klonalität

  • 439
  • 51
Monoklonal

Konjugat

  • 209
  • 33
  • 27
  • 26
  • 16
  • 15
  • 10
  • 10
  • 10
  • 10
  • 10
  • 10
  • 10
  • 10
  • 10
  • 10
  • 10
  • 9
  • 9
  • 9
  • 9
  • 9
  • 9
Dieser RAF1 Antikörper ist unkonjugiert

Applikation

  • 299
  • 207
  • 104
  • 104
  • 95
  • 73
  • 44
  • 34
  • 28
  • 24
  • 17
  • 16
  • 16
  • 3
  • 2
  • 2
  • 1
Western Blotting (WB), ELISA, Immunofluorescence (IF)

Klon

1C2
  • Bindungsspezifität

    • 49
    • 48
    • 35
    • 33
    • 27
    • 24
    • 24
    • 24
    • 23
    • 20
    • 19
    • 15
    • 15
    • 14
    • 12
    • 8
    • 8
    • 8
    • 8
    • 6
    • 6
    • 6
    • 5
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    pSer621

    Kreuzreaktivität

    Human

    Aufreinigung

    Affinity-chromatography

    Immunogen

    A synthesized peptide derived from human Phospho-RAF1 (S621)

    Isotyp

    IgG
  • Applikationshinweise

    Recommended dilution: WB:1:500-1:5000, IF:1:20-1:200,

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Liquid

    Buffer

    Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Lagerung

    -20 °C,-80 °C

    Informationen zur Lagerung

    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • Target

    RAF1 (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1))

    Andere Bezeichnung

    RAF1

    Hintergrund

    Background: Serine/threonine-protein kinase that acts as a regulatory link between the membrane-associated Ras GTPases and the MAPK/ERK cascade, and this critical regulatory link functions as a switch determining cell fate decisions including proliferation, differentiation, apoptosis, survival and oncogenic transformation. RAF1 activation initiates a mitogen-activated protein kinase (MAPK) cascade that comprises a sequential phosphorylation of the dual-specific MAPK kinases (MAP2K1/MEK1 and MAP2K2/MEK2) and the extracellular signal-regulated kinases (MAPK3/ERK1 and MAPK1/ERK2). The phosphorylated form of RAF1 (on residues Ser-338 and Ser-339, by PAK1) phosphorylates BAD/Bcl2-antagonist of cell death at 'Ser-75'. Phosphorylates adenylyl cyclases: ADCY2, ADCY5 and ADCY6, resulting in their activation. Phosphorylates PPP1R12A resulting in inhibition of the phosphatase activity. Phosphorylates TNNT2/cardiac muscle troponin T. Can promote NF-kB activation and inhibit signal transducers involved in motility (ROCK2), apoptosis (MAP3K5/ASK1 and STK3/MST2), proliferation and angiogenesis (RB1). Can protect cells from apoptosis also by translocating to the mitochondria where it binds BCL2 and displaces BAD/Bcl2-antagonist of cell death. Regulates Rho signaling and migration, and is required for normal wound healing. Plays a role in the oncogenic transformation of epithelial cells via repression of the TJ protein, occludin (OCLN) by inducing the up-regulation of a transcriptional repressor SNAI2/SLUG, which induces down-regulation of OCLN. Restricts caspase activation in response to selected stimuli, notably Fas stimulation, pathogen-mediated macrophage apoptosis, and erythroid differentiation.

    Aliases: RAF proto-oncogene serine/threonine-protein kinase, Proto-oncogene c-RAF, cRaf, Raf-1, RAF1, RAF

    UniProt

    P04049

    Pathways

    MAPK Signalweg, RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, Neurotrophin Signalübertragung, cAMP Metabolic Process, Stem Cell Maintenance, Hepatitis C, Autophagie, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling, BCR Signaling
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