ITGAV/ITGB6 Antikörper

Produktnummer ABIN7127264

Produktdetails anti-ITGAV/ITGB6 Antikörper

Target: ITGAV/ITGB6 (Integrin AlphaV Beta6 (ITGAV/ITGB6))

Reaktivität: Human

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser ITGAV/ITGB6 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Western Blotting (WB), ELISA, Immunoprecipitation (IP), Coating (Coat), Immunofluorescence (IF)

Spezifität: Integrins are heterodimers composed of noncovalently associated transmembrane i± and i² subunits. The 16 i± and 8 i² subunits heterodimerize to produce more than 20 different receptors. Most integrin receptors bind ligands that are components of the extracellular matrix, including Fibronectin, Collagen and Vitronectin. Certain integrins can also bind to soluble ligands such as Fibrinogen, or to counterreceptors on adjacent cells such as the intracellular adhesion molecules (ICAMs), leading to aggregation of cells. Ligands serve to cross-link or cluster integrins by binding to adjacent integrin receptors, both receptor clustering and ligand occupancy are necessary for the activation of integrin-mediated responses. In addition to mediating cell adhesion and cytoskeletal organization, integrins function as signaling receptors. Signals transduced by integrins play a role in many biological processes, including cell growth, differentiation, migration and apoptosis.

Kreuzreaktivität (Details): Human.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Mouse fibroblasts retrovirally infected with cDNA to human beta6, Cells expressed a mouse/human avb6 chimera.

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise:

Known_Application: ELISA (For coating use Ab at 1-5 μg/mL, order Ab without BSA), Immunoprecipitation (1-2 μg/500 μg protein), ,Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (0.5-1 μg/mL),Optimal dilution for a specific application should be determined.

Positive_Control: Squamous cell carcinoma cells. A375M melanoma cell line. A549 cell line.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu ITGAV/ITGB6

Target: ITGAV/ITGB6 (Integrin AlphaV Beta6 (ITGAV/ITGB6))

Andere Bezeichnung: ITGAV & ITGB6 (ITGAV/ITGB6 Produkte)

Hintergrund: Antigen identified by monoclonal antibody L230, CD 51, CD51, Integrin alpha five, integrin alpha V beta 3, Integrin alpha-V light chain, integrin alphaVbeta3, integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51), ITGAV, MSK 8, Msk8, Vitronectin receptor subunit alpha, VNRA, VTNR, ITGB6, Integrin, beta 6,Integrin alpha V + beta 6
Cellular localisation: Membrane.

Molekulargewicht: 150-160kDa under non-reducing conditions. Under reducing conditions, cleaved into 2 bands of ~125-130kDa +20-25kDa

Gen-ID: 3685, 3694, 436873

UniProt: P06756, P18564