SLC5A1 Antikörper (Internal Region)

Produktnummer ABIN7013885

Produktdetails anti-SLC5A1 Antikörper

Target: SLC5A1 (Solute Carrier Family 5 (Sodium/glucose Cotransporter), Member 1 (SLC5A1))

Bindungsspezifität: Internal Region

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser SLC5A1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Verwendungszweck: SGLT1

Sequenz: KETIEIETQV PEKKK

Kreuzreaktivität: Human

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Güteklasse: Verified

Anwendungsinformationen

Applikationshinweise:

DS WB Results: Approx 75 kDa band observed in isolated Brush Border membranes from Human Kidney (calculated MW of 73.5 kDa according to NP_000334.1). Recommended concentration: 0.3-1 μg/mL. Primary incubation was 1 hour.

DS IHC Results: In paraffin embedded Human Small Intestine shows staining of the glycocalix. Recommended concentration: 3-6 μg/mL.

Peptide ELISA Statement: Peptide ELISA: antibody detection limit dilution 1:4000.

Kommentare:

Immunocytochemistry: Fig A.Frozen sections of Human Kidney show staining of the brush borders in DCT, while only weak background was observed in Rat and Pig Kidney. Recommended concentration: 2-4ug/ml. Fig B.PFA-fixed 4μm cryosections of Human Kidney shows staining of the brush border of proximal straight tubules (S3 segments) in the outer stripe zone. The signal drops to background levels upon blocking with the immunizing peptide.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin. Aliquot and store at -20 °C. Minimize freezing and thawing.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Details zu SLC5A1

Target: SLC5A1 (Solute Carrier Family 5 (Sodium/glucose Cotransporter), Member 1 (SLC5A1))

Andere Bezeichnung: SLC5A1 (SLC5A1 Produkte)

Synonyme: D22S675 antikoerper, NAGT antikoerper, SGLT1 antikoerper, zgc:66074 antikoerper, zgc:85782 antikoerper, SLC5A1 antikoerper, Sglt1 antikoerper, solute carrier family 5 member 1 antikoerper, solute carrier family 5 (sodium/glucose cotransporter), member 1 antikoerper, SLC5A1 antikoerper, slc5a1 antikoerper, Slc5a1 antikoerper

Hintergrund: SLC5A1, solute carrier family 5 (sodium/glucose cotransporter), member 1, D22S675, NAGT, SGLT1, Human Na+/glucose cotransporter 1 mRNA, complete cds, sodium/glucose cotransporter 1, solute carrier family 5 (sodium/glucose transporter), member 1

Gen-ID: 6523