Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Spezifität
TRPC3 antibody is human, mouse and rat reactive. At least four isoforms are known to exist, this antibody will detect the three largest isoforms. TRPC3 antibody is predicted to not cross-react with TRPC6.
Aufreinigung
TRPC3 Antibody is affinity chromatography purified via peptide column.
Immunogen
TRPC3 antibody was raised against a 19 amino acid peptide from near the carboxy terminus of human TRPC3. The immunogen is located within amino acids 710 - 760 of TRPC3.
TRPC3 antibody can be used for the detection of TRPC3 by Western blot at 1 - 2 μ,g/mL.Antibody can also be used for immunohistochemistry starting at 2.5 μ,g/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
1 mg/mL
Buffer
TRPC3 Antibody is supplied in PBS containing 0.02 % sodium azide.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
-20 °C,4 °C
Informationen zur Lagerung
TRPC3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
The mammalian transient receptor potential (TRP) superfamily can be divided into three major families including the "canonical TRP" (TRPC) family. The seven members of this family share the activation through PLC-coupled receptors and have been suggested to be components of receptor-regulated cation channels in different cell types. Furthermore, the members of the TRPC3/6/7 subfamily can be activated by diacylglycerol analogs, suggesting a possible mechanism of activation of these channels by PLC-coupled receptors. TRPC3 encodes a Ca2+-permeant channel that is agonist-activated but not store-operated or directly receptor-activated. TRPC3 physically interacts with TRPC6 and TRPC7 and forms functional tetrameric channels.