PJA1 Antikörper (N-Term)

Produktnummer ABIN6992094

Produktdetails anti-PJA1 Antikörper

Target: PJA1 (Praja Ring Finger 1, E3 Ubiquitin Protein Ligase (PJA1))

Bindungsspezifität: AA 50-100, N-Term

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PJA1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Spezifität: PJA1 antibody is human, mouse and rat reactive. At least two isoforms of PJA1 are known to exist, this antibody will detect both isoforms.

Aufreinigung: PJA1 antibody is affinity chromatography purified via peptide column.

Immunogen: PJA1 antibody was raised against a 19 amino acid peptide near the amino terminus of human PJA1. The immunogen is located within amino acids 50 - 100 of PJA1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: PJA1 antibody can be used for detection of PJA1 by Western blot at 0.25 - 0.5 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PJA1 antibody is supplied in PBS containing 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: PJA1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Details zu PJA1

Target: PJA1 (Praja Ring Finger 1, E3 Ubiquitin Protein Ligase (PJA1))

Andere Bezeichnung: PJA1 (PJA1 Produkte)

Hintergrund: Ubiquitinization is an important cellular degradation process requiring sequential reactions that are mediated by three enzymes: E1, E2 and E3. PJA1, also known as Praja1 and RING finger protein 70, is a 643 amino acid E2-dependent E3-ubiquitin ligase that is abundantly expressed in the brain (1,2). Through interaction and activation with the E2-ubiquitin ligase UBC4, PJA1 mediates substrate-specific ubiquitination via its RING finger domain and facilitates ubiquitination (3). Overexpression of PJA1 in gastrointestinal cancers suggests that it may be responsible for the degradation of some anti-oncogenic proteins (4,5).

Molekulargewicht: Predicted: 71 kDa

Observed: 71 kDa

Gen-ID: 64219

NCBI Accession: NP_660095

UniProt: Q8NG27